Mechanics of sperm entry in cycling hamsters

Ruth Shalgi*, David Phillips

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


At various times after mating, fertilized eggs were flushed from the ampulla of the oviduct of estrus hamsters. Cumulus cells were removed with hyaluronidase before fixation. After fixation, the zonae pellucidae were removed mechanically. The initial association between the fertilizing spermatozoon and the oocyte surface appears to be between the tips of oocyte microvilli and the equatorial segment. As the sperm head is incorporated, it undergoes a pronounced bend such that the anterior tip is the last portion of the sperm to be incorporated. A small incorporation cone forms at the site where the sperm head was incorporated into the ooplasm. As the sperm tail is incorporated, it forms numerous bends which distort the oolemma into a row of small elevations. From these observations, we have been able to propose a model for the mechanics of sperm entry into the ovum in vivo.

Original languageEnglish
Pages (from-to)154-161
Number of pages8
JournalJournal of Structural Biology
Issue number2
StatePublished - May 1980
Externally publishedYes


FundersFunder number
Rockefeller Foundation


    Dive into the research topics of 'Mechanics of sperm entry in cycling hamsters'. Together they form a unique fingerprint.

    Cite this