TY - JOUR
T1 - Measurement of Microbial Adhesion to Hydrophobic Substrata
AU - Doyle, Ron J.
AU - Rosenberg, Mel
N1 - Funding Information:
The authors have been supported by National Institutes of Health Grants DE-07199 and DE-05102 and by the U.S.-Israel BiNational Science Foundation.
Funding Information:
Portions of the work on methods development have been supported by the National Institutes of Health, NIDR DE-05102 and DE-07199.
PY - 1995
Y1 - 1995
N2 - This chapter summarizes the most commonly employed method in determining adhesion to hydrophobins. The microbial adhesion to hydrocarbon (MATH) assay is based on the tendency of certain microorganisms to adhere to the surfaces of liquid hydrocarbon droplets, during a brief mixing period. The assay can be performed directly within a cuvette or tube that is compatible with a spectrophotometer, in which case the decrease in turbidity can be determined directly, provided that (1) the hydrocarbon droplets have risen completely from the aqueous phase, (2) care is taken to ensure that the upper phase does not interfere with the light beam, and (3) the walls of the vessel are free of interfering droplets. There are numerous factors involved in adhesion to hydrophobins, such as pH, ionic strength, temperature, age of cells, growth medium for the cells, time of incubation (or vortexing), antibiotics, and others. There is a good correlation among MATH and the other assays. For this reason and the fact that the method is simple and reproducible, MATH has become the assay of choice. The chapter also outlines other methods commonly employed to measure microbial cell surface hydrophobicity and adhesion to hydrophobins.
AB - This chapter summarizes the most commonly employed method in determining adhesion to hydrophobins. The microbial adhesion to hydrocarbon (MATH) assay is based on the tendency of certain microorganisms to adhere to the surfaces of liquid hydrocarbon droplets, during a brief mixing period. The assay can be performed directly within a cuvette or tube that is compatible with a spectrophotometer, in which case the decrease in turbidity can be determined directly, provided that (1) the hydrocarbon droplets have risen completely from the aqueous phase, (2) care is taken to ensure that the upper phase does not interfere with the light beam, and (3) the walls of the vessel are free of interfering droplets. There are numerous factors involved in adhesion to hydrophobins, such as pH, ionic strength, temperature, age of cells, growth medium for the cells, time of incubation (or vortexing), antibiotics, and others. There is a good correlation among MATH and the other assays. For this reason and the fact that the method is simple and reproducible, MATH has become the assay of choice. The chapter also outlines other methods commonly employed to measure microbial cell surface hydrophobicity and adhesion to hydrophobins.
UR - http://www.scopus.com/inward/record.url?scp=0029060723&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(95)53046-0
DO - 10.1016/S0076-6879(95)53046-0
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AN - SCOPUS:0029060723
SN - 0076-6879
VL - 253
SP - 542
EP - 550
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -