Meal intake increases circulating procoagulant microparticles in patients with type 1 and type 2 diabetes mellitus

Galia Spectre, Fariborz Mobarrez, Ragnhild Stålesen, Claes Göran Östenson, David Varon, Håkan Wallen, Paul Hjemdahl*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Diabetes mellitus (DM) is associated with prothrombotic alterations, and postprandial hyperglycemia is an independent risk factor for cardiovascular complications. We therefore investigated whether a standardized mixed meal alters circulating microparticles (MPs) and their procoagulant activity in DM patients. Patients with DM type 1 (T1DM, n = 11) and type 2 (T2DM; n = 9) were studied before and 90 min after a standardized meal (without premeal insulin). MPs in plasma derived from platelets (PMPs), endothelial cells (EMPs), or monocytes (MMPs) were measured by flow cytometry. MP-induced thrombin generation in plasma was assessed by a calibrated automated thrombogram. In the fasting state, MPs did not differ significantly between T1DM and T2DM. Meal intake increased the following microparticles: PMPs expressing phosphatidylserine (by 55%, on average), P-selectin (by 86%), and tissue factor (TF; by 112%); EMPs expressing E-selectin (by 96%) and MMPs expressing TF (by 164%), with no significant group differences between T1DM and T2DM. There were no increments in EMPs expressing phosphatidylserine or TF. Meal intake increased MP-induced thrombin generation similarly in T1DM and T2DM with increased endogenous thrombin potential (p = 0.02) and peak thrombin (p = 0.03) and shortened time to peak (p = 0.02). Phosphatidylserine inhibition by lactadherin completely abolished MP-induced thrombin generation, while an anti-TF antibody had no effect. In conclusion, meal intake increased several types of circulating MPs in patients with diabetes mellitus. These MPs have a procoagulant potential, which is related to phosphatidylserine expression and negatively charged MP surfaces rather than to TF.

Original languageEnglish
Pages (from-to)348-355
Number of pages8
JournalPlatelets
Volume30
Issue number3
DOIs
StatePublished - 3 Apr 2019

Funding

FundersFunder number
Hjärt-Lungfonden
Karolinska Institutet
Stockholms Läns Landsting

    Keywords

    • Diabetes mellitus
    • microparticles
    • postprandial
    • thrombin generation

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