TY - JOUR
T1 - Mathematical model of whole cell based bio-chip
T2 - An electrochemical biosensor for water toxicity detection
AU - Popovtzer, Rachela
AU - Natan, Amir
AU - Shacham-Diamand, Yosi
PY - 2007/4/1
Y1 - 2007/4/1
N2 - Whole cell biosensors, which have been genetically engineered to respond to environmental stress, trigger a sequence of processes, which leads to generation of electrical current. This work presents a mathematical model describing the kinetic properties of the bacterial enzymatic reactions in response to toxic chemicals, and the resulting electro-active molecule diffusion to the electrode in a miniaturized electrochemical cell. The model characterizes the generated electrical current as a function of bacteria and toxicant concentrations, electrochemical cell dimensions and electrode dimensions. A model was framed for a 100 nL spherical electrochemical cell. Its performance was simulated and compared to experimental data. This simulation results agreed well with the measured data and therefore it should be useful in predicting current variations in similar systems with different geometries, materials and biological components.
AB - Whole cell biosensors, which have been genetically engineered to respond to environmental stress, trigger a sequence of processes, which leads to generation of electrical current. This work presents a mathematical model describing the kinetic properties of the bacterial enzymatic reactions in response to toxic chemicals, and the resulting electro-active molecule diffusion to the electrode in a miniaturized electrochemical cell. The model characterizes the generated electrical current as a function of bacteria and toxicant concentrations, electrochemical cell dimensions and electrode dimensions. A model was framed for a 100 nL spherical electrochemical cell. Its performance was simulated and compared to experimental data. This simulation results agreed well with the measured data and therefore it should be useful in predicting current variations in similar systems with different geometries, materials and biological components.
KW - Electrochemical biosensor
KW - Enzyme biosensor
KW - Model
KW - Water toxicity
UR - http://www.scopus.com/inward/record.url?scp=33847670236&partnerID=8YFLogxK
U2 - 10.1016/j.jelechem.2006.11.022
DO - 10.1016/j.jelechem.2006.11.022
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:33847670236
SN - 1572-6657
VL - 602
SP - 17
EP - 23
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
IS - 1
ER -