Maternal diet enriched with α-linolenic or saturated fatty acids differentially regulates gene expression in the liver of mouse offspring

Alicia Leikin-Frenkel, Limor Shomonov-Wagner, Ana Juknat, Metsada Pasmanik-Chor

Research output: Contribution to journalArticlepeer-review

Abstract

Background/Aims: Lipid metabolic disarray in the liver of young and adult mice offspring is induced by saturated fatty acids (SFA) but prevented by α-linolenic acid (ALA, 18:3 ω-3) in the maternal diet during pregnancy and lactation. The aim of the present study was to analyze the impact of maternal dietary ALA compared to an SFA diet on the liver gene expression in the newborn offspring. Methods: C57Bl6/J dams were fed with diets normal in calories but rich in ALA or SFA before mating and during pregnancy. Pups were sacrificed at birth and liver parameters were assessed. Gene expression was characterized by microarray analysis and validated by real-time quantitative PCR. Results: ALA, compared to SFA, in maternal diets during pregnancy increased polyunsaturated fatty acids, while it differentially modified fatty acid desaturase activities in offspring liver. Overall, 474 and 662 genes from the liver of newborn pups were differentially regulated by ALA and SFA compared to control diet (p < 0.05; fold change 2), respectively. Notably, Per3 was upregulated by ALA, whereas it was downregulated by SFA, compared to control diet. Conclusions: ALA- and SFA-enriched diets differentially affect the gene expression pattern in the offspring's liver. ALA, in particular, upregulates genes associated with low adiposity.

Original languageEnglish
Pages (from-to)185-194
Number of pages10
JournalJournal of Nutrigenetics and Nutrigenomics
Volume8
Issue number4-6
DOIs
StatePublished - 1 Apr 2016

Keywords

  • Chronic disease
  • Fatty acids
  • Fetal programming
  • Gene expression
  • Maternal diet
  • Microarray

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