TY - JOUR
T1 - Marrow adipocytes regulate growth and differentiation of osteoblasts
AU - Benayahu, D.
AU - Zipori, D.
AU - Wientroub, S.
PY - 1993
Y1 - 1993
N2 - Conditioned media (CM) from various marrow subpopulations (MBA series) were examined for their effects on growth and maturation of marrow-derived osteoblastic cells, MBA-15. CM harvested from a stromal adipocytes, 14F1.1, stimulated cell growth, collagen but not non-collagenous proteins synthesized by MBA-15 cells. Alkaline phosphatase activity was inhibited under the same culture conditions. These results were associated with morphological changes, i.e., the large cuboidal MBA-15 cells acquired a fibroblast-like appearance. Exposure of MBA-15 cells to known growth factors: BMP-2, TGFβ, IGF-I and PDGF or combined with 14F1.1 CM resulted in a dominant effect of the latter. This may imply that marrow adipocytes produce factor/s that participate in the stromal regulation of osteoblastic functions.
AB - Conditioned media (CM) from various marrow subpopulations (MBA series) were examined for their effects on growth and maturation of marrow-derived osteoblastic cells, MBA-15. CM harvested from a stromal adipocytes, 14F1.1, stimulated cell growth, collagen but not non-collagenous proteins synthesized by MBA-15 cells. Alkaline phosphatase activity was inhibited under the same culture conditions. These results were associated with morphological changes, i.e., the large cuboidal MBA-15 cells acquired a fibroblast-like appearance. Exposure of MBA-15 cells to known growth factors: BMP-2, TGFβ, IGF-I and PDGF or combined with 14F1.1 CM resulted in a dominant effect of the latter. This may imply that marrow adipocytes produce factor/s that participate in the stromal regulation of osteoblastic functions.
UR - http://www.scopus.com/inward/record.url?scp=0027752753&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.2611
DO - 10.1006/bbrc.1993.2611
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AN - SCOPUS:0027752753
SN - 0006-291X
VL - 197
SP - 1245
EP - 1252
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -