Manno(rhamno)biose-containing capsular polysaccharides of klebsiella pneumoniae enhance opsono-stimulation of human polymorphonuclear leukocytes

Hany Sahly*, Yona Keisari, Itzhak Ofek

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


We tested the relationship between the capsular and the O-antigen structures and the ability of bacteria to trigger respiratory burst in human polymorphonuclear leukocytes (PMNL).Capsulated and non-capsulated variants as well as capsule-switched derivatives of Klebsiella serotypes bearing or lacking manno(rhamno)biose repeats in their capsular polysaccharides and expressing either mannose-rich or mannose-poor O antigens were tested for their ability to induce respiratory burst and survive in human PMNL. Luminol-enhanced chemiluminescence (CL) was measured to quantify respiratory burst. Intracellular survival was quantified by determining the viable counts of intracellular bacteria. K serotypes and the capsule-switched derivative lacking manno(rhamno)biose induced significantly lower CL than those expressing manno(rhamno)biose. Manno(rhamno)biose-lacking serotypes survived in the cells significantly better than serotypes expressing these repeats. C1q depletion did not affect CL induced by the manno(rhamno)biose-containing serotype, whereas factor B depletion revealed a significantly reduced CL. Likewise, EGTA in the presence of Mg 2+ significantly decreased CL, but the values were higher than those induced by the bacterium opsonized with factor B-depleted serum. In the presence of EGTA, Mg 2+-treated factor B-depleted serum revealed a significant reduction in the CL response compared with the responses induced by opsonization with factor B-depleted serum alone. These results indicate, in addition to the alternative pathway, a manno(rhamno)biose pattern recognition of Klebsiella by PMNL probably by the complement lectin pathway.

Original languageEnglish
Pages (from-to)136-144
Number of pages9
JournalJournal of Innate Immunity
Issue number2
StatePublished - Jan 2009


  • Capsular polysaccharides
  • Complement
  • Innate immunity
  • Klebsiella
  • Lipopolysaccharides
  • Mannose-binding lectin
  • Polymorphonuclear leukocytes
  • Respiratory burst


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