Mammalian meiosis involves DNA double-strand breaks with 3′ overhangs

Drora Zenvirth, Carmelit Richler, Amit Bardhan, Frédéric Baudat, Ari Barzilai, Amit Wahrman, Giora Simchen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Meiotic recombination in yeast is initiated at DMA double-strand breaks (DSBs), processed into 3′ single-strand overhangs that are active in homology search, repair and formation of recombinant molecules. Are 3′ overhangs recombination intermediaries in mouse germ cells too? To answer this question we developed a novel approach based on the properties of the Klenow enzyme. We carried out two different, successive in situ Klenow enzyme-based reactions on sectioned preparations of testicular tubules. Signals showing 3′ overhangs were observed during wild-type mouse spermatogenesis, but not in Spo11-/- males, which lack meiotic DSBs. In Atm-/- mice, abundant positively stained spermatocytes were present, indicating an accumulation of non-repaired DSBs, suggesting the involvement of ATM in repair of meiotic DSBs. Thus the processing of DSBs into 3′ overhangs is common to meiotic cells in mammals and yeast, and probably in all eukaryotes.

Original languageEnglish
Pages (from-to)369-376
Number of pages8
Issue number6
StatePublished - Mar 2003


FundersFunder number
USA/Israel Binational Science Foundation
Charles H. Revson Foundation
Lady Davis Fellowship Trust, Hebrew University of Jerusalem
United States-Israel Binational Science Foundation


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