Mammalian DNA enriched for replication origins is enriched for snap-back sequences

Maria Zannis-Hadjopoulos, Gabriel Kaufmann, Robert G. Martin

Research output: Contribution to journalArticlepeer-review

Abstract

Using the instability of replication loops as a method for the isolation of double-stranded nascent DNA, extruded DNA enriched for replication origins was obtained and denatured. Snap-back DNA, single-stranded DNA with inverted repeats (palindromic sequences), reassociates rapidly into stem-loop structures with zero-order kinetics when conditions are changed from denaturing to renaturing, and can be assayed by chromatography on hydroxyapatite. Origin-enriched nascent DNA strands from mouse, rat and monkey cells growing either synchronously or asynchronously were purified and assayed for the presence of snap-back sequences. The results show that origin-enriched DNA is also enriched for snap-back sequences, implying that some origins for mammalian DNA replication contain or lie near palindromic sequences.

Original languageEnglish
Pages (from-to)577-586
Number of pages10
JournalJournal of Molecular Biology
Volume179
Issue number4
DOIs
StatePublished - 15 Nov 1984

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