TY - JOUR
T1 - Mammalian DNA enriched for replication origins is enriched for snap-back sequences
AU - Zannis-Hadjopoulos, Maria
AU - Kaufmann, Gabriel
AU - Martin, Robert G.
PY - 1984/11/15
Y1 - 1984/11/15
N2 - Using the instability of replication loops as a method for the isolation of double-stranded nascent DNA, extruded DNA enriched for replication origins was obtained and denatured. Snap-back DNA, single-stranded DNA with inverted repeats (palindromic sequences), reassociates rapidly into stem-loop structures with zero-order kinetics when conditions are changed from denaturing to renaturing, and can be assayed by chromatography on hydroxyapatite. Origin-enriched nascent DNA strands from mouse, rat and monkey cells growing either synchronously or asynchronously were purified and assayed for the presence of snap-back sequences. The results show that origin-enriched DNA is also enriched for snap-back sequences, implying that some origins for mammalian DNA replication contain or lie near palindromic sequences.
AB - Using the instability of replication loops as a method for the isolation of double-stranded nascent DNA, extruded DNA enriched for replication origins was obtained and denatured. Snap-back DNA, single-stranded DNA with inverted repeats (palindromic sequences), reassociates rapidly into stem-loop structures with zero-order kinetics when conditions are changed from denaturing to renaturing, and can be assayed by chromatography on hydroxyapatite. Origin-enriched nascent DNA strands from mouse, rat and monkey cells growing either synchronously or asynchronously were purified and assayed for the presence of snap-back sequences. The results show that origin-enriched DNA is also enriched for snap-back sequences, implying that some origins for mammalian DNA replication contain or lie near palindromic sequences.
UR - http://www.scopus.com/inward/record.url?scp=0021684862&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(84)90156-6
DO - 10.1016/0022-2836(84)90156-6
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AN - SCOPUS:0021684862
SN - 0022-2836
VL - 179
SP - 577
EP - 586
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 4
ER -