TY - JOUR
T1 - Male and female genomes associated in a single pronucleus in human zygotes
AU - Levron, J.
AU - Munne, S.
AU - Willadsen, S.
AU - Rosenwaks, Z.
AU - Cohen, J.
PY - 1995
Y1 - 1995
N2 - The ploidy of single-pronucleated human zygotes obtained after conventional in vitro fertilization was determined by fluorescent in situ hybridization (FISH) using multiple simultaneous probes for gonosomes and autosomes. After zona removal the single-pronucleated zygotes were exposed to cytochalasin B, and the pronucleus, surrounded by scant cytoplasm and the plasma membrane (karyoplast), was divided from the rest of the egg (cytoplast). The karyoplasts and the corresponding cytoplasts were analyzed separately by FISH. Of the 16 zygotes analyzed, 10 had haploid pronuclei and 6 were diploid. Four diploid pronuclei contained XY chromosomes, and 2 contained XX chromosomes. These results suggest that during the course of their interaction, human gamete nuclei can associate together and form diploid, single-pronucleated zygotes. These findings confirm a newly recognized variation of human pronuclear interaction during syngamy.
AB - The ploidy of single-pronucleated human zygotes obtained after conventional in vitro fertilization was determined by fluorescent in situ hybridization (FISH) using multiple simultaneous probes for gonosomes and autosomes. After zona removal the single-pronucleated zygotes were exposed to cytochalasin B, and the pronucleus, surrounded by scant cytoplasm and the plasma membrane (karyoplast), was divided from the rest of the egg (cytoplast). The karyoplasts and the corresponding cytoplasts were analyzed separately by FISH. Of the 16 zygotes analyzed, 10 had haploid pronuclei and 6 were diploid. Four diploid pronuclei contained XY chromosomes, and 2 contained XX chromosomes. These results suggest that during the course of their interaction, human gamete nuclei can associate together and form diploid, single-pronucleated zygotes. These findings confirm a newly recognized variation of human pronuclear interaction during syngamy.
UR - http://www.scopus.com/inward/record.url?scp=0028897106&partnerID=8YFLogxK
U2 - 10.1095/biolreprod52.3.653
DO - 10.1095/biolreprod52.3.653
M3 - מאמר
C2 - 7756458
AN - SCOPUS:0028897106
VL - 52
SP - 653
EP - 657
JO - Biology of Reproduction
JF - Biology of Reproduction
SN - 0006-3363
IS - 3
ER -