Magnetization transfer based contrast for imaging denatured collagen

Amir Harel, Uzi Eliav, Solange Akselrod, Gil Navon

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To study of the sensitivity of various NMR and MRI methods and parameters to the degree of thermal denaturation of collagen. Materials and Methods: Collagen type I powder was washed with methanohchloroform to remove traces of lipids and then suspended in saline. Denaturation was carried out by heating the suspension for 5-120 minutes at a temperature range of 50-100°C. The NMR methods tested were two T2 filter methods: Goldman-Shen (GS) and Edzes-Samulski (ES); magnetization transfer contrast (MTC); double quantum filtering (DQF) and high resolution spectroscopy. MRI contrasts based on these methods were compared. Results: The following parameters were found to be sensitive to denaturation of collagen: 1) the amount of spins that experience high dipolar interactions as assessed by DQF; 2) MTR; 3) kwT 1w (where kw is the magnetization transfer rate from water to collagen, and T1w is the water protons longitudinal relaxation time); and 4) aliphatic residues content. The contrast between native and denatured collagen was improved by all the tested methods, with ES and DQF producing the highest contrast. Conclusion: Methods depending on T2 filtering and DQF were found to be sensitive to the degree of thermal denaturation of collagen and improve the contrast between native and denatured collagen.

Original languageEnglish
Pages (from-to)1155-1163
Number of pages9
JournalJournal of Magnetic Resonance Imaging
Volume27
Issue number5
DOIs
StatePublished - May 2008

Keywords

  • Collagen
  • Denaturation
  • Double quantum filtering
  • MRI
  • Magnetization transfer
  • NMR

Fingerprint

Dive into the research topics of 'Magnetization transfer based contrast for imaging denatured collagen'. Together they form a unique fingerprint.

Cite this