TY - JOUR
T1 - Magnesium ion dependence of free and polysomal ribosomes from Escherichia coli
AU - Ron, Eliora Z.
AU - Kohler, Robert E.
AU - Davis, Bernard D.
N1 - Funding Information:
This work was supported by research grant GB1307 from the National Science Foundation. We thank Miss G. Elizabeth Wylde for excellent technical assistance.
PY - 1968/8/28
Y1 - 1968/8/28
N2 - This paper presents a simple means of distinguishing two kinds of 70 s ribosomes from Escherichia coli: intact complexes with mRNA and peptidyl-tRNA, obtained by ribonuclease treatment of polysomes; and free ribosomes, relieved of the polypeptide (and presumably of tRNA and mRNA) by treatment with puromycin in vitro. Free ribosomes are completely dissociated into subunits at 1 to 2 mm-Mg2+, while preparations containing polysomal ribosomes are only partly dissociated. Evidence obtained by the application of this criterion supports the conclusion, presented in the preceding paper, that the cell normally contains undissociated free ribosomes, and that their concentration increases when the products of polysomes runoff are caused to accumulate.
AB - This paper presents a simple means of distinguishing two kinds of 70 s ribosomes from Escherichia coli: intact complexes with mRNA and peptidyl-tRNA, obtained by ribonuclease treatment of polysomes; and free ribosomes, relieved of the polypeptide (and presumably of tRNA and mRNA) by treatment with puromycin in vitro. Free ribosomes are completely dissociated into subunits at 1 to 2 mm-Mg2+, while preparations containing polysomal ribosomes are only partly dissociated. Evidence obtained by the application of this criterion supports the conclusion, presented in the preceding paper, that the cell normally contains undissociated free ribosomes, and that their concentration increases when the products of polysomes runoff are caused to accumulate.
UR - http://www.scopus.com/inward/record.url?scp=0014433087&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(68)90221-0
DO - 10.1016/0022-2836(68)90221-0
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AN - SCOPUS:0014433087
SN - 0022-2836
VL - 36
SP - 83
EP - 89
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -