TY - JOUR
T1 - Macrophage-mediated cytolysis of erythrocytes in the guinea pig. I. Activation by stimulators of the oxidative burst
AU - Keisari, Yona
AU - Pick, Edgar
PY - 1981/7/15
Y1 - 1981/7/15
N2 - Oxygen metabolites generated by macrophages may exert membrane injury to various cells. In this study reagents, which induce superoxide (O2-) and hydrogen peroxide (H2O2) production by paraffin oil elicited adherence purified guinea pig peritoneal macrophages (GPPM), were studied as to their potential to activate macrophage-mediated cytolysis (MMC) against allogeneic and autologous erythrocytes. Strong MMC reactions were activated by 12-O-tetra-decanoylphorbol-13-acetate (TPA), methylated TPA (4-O-MeTPA), opsonized zymosan, and out of six lectins tested, by wheat germ agglutinin (WGA) and concanavalin A (Con A). The cGMP elevators: sodium nitroprusside and sodium azide and the formyl-methionyl-type chemotactic peptides were ineffective. MMC activated by TPA, 4-O-MeTPA, WGA, and Con A was unaffected by colchicine and partially inhibited by cytochalasin B. TPA-activated MMC was abolished by diethyldithiocarbamate (DDC) (inhibitor of superoxide dismutase) and catalase, while WGA and Con A-activated MMC were only partially inhibited by DDC and unaffected by catalase.
AB - Oxygen metabolites generated by macrophages may exert membrane injury to various cells. In this study reagents, which induce superoxide (O2-) and hydrogen peroxide (H2O2) production by paraffin oil elicited adherence purified guinea pig peritoneal macrophages (GPPM), were studied as to their potential to activate macrophage-mediated cytolysis (MMC) against allogeneic and autologous erythrocytes. Strong MMC reactions were activated by 12-O-tetra-decanoylphorbol-13-acetate (TPA), methylated TPA (4-O-MeTPA), opsonized zymosan, and out of six lectins tested, by wheat germ agglutinin (WGA) and concanavalin A (Con A). The cGMP elevators: sodium nitroprusside and sodium azide and the formyl-methionyl-type chemotactic peptides were ineffective. MMC activated by TPA, 4-O-MeTPA, WGA, and Con A was unaffected by colchicine and partially inhibited by cytochalasin B. TPA-activated MMC was abolished by diethyldithiocarbamate (DDC) (inhibitor of superoxide dismutase) and catalase, while WGA and Con A-activated MMC were only partially inhibited by DDC and unaffected by catalase.
UR - http://www.scopus.com/inward/record.url?scp=0019413821&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(81)90311-7
DO - 10.1016/0008-8749(81)90311-7
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AN - SCOPUS:0019413821
SN - 0008-8749
VL - 62
SP - 172
EP - 185
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -
