TY - JOUR
T1 - Lymphoproliferative disease virus of turkeys
T2 - sequence analysis and transcriptional activity of the long terminal repeat
AU - Gak, Eva
AU - Yaniv, Abraham
AU - Sherman, Levana
AU - Ianconescu, Marius
AU - Tronick, Steven R.
AU - Gazit, Arnona
N1 - Funding Information:
We thank Dr. S.A. Aaronson for his continued support and encouragement.T his study was supportedb y a Project Grant from the Fund for Basic Research administrated by the Israel Academy of Sciences and Humanities, the Israel Cancer Research Fund and the Israel Cancer Association.
PY - 1991/3/15
Y1 - 1991/3/15
N2 - The lymphoproliferative disease virus (LPDV) is the etiological agent of a lymphoproliferative disease that naturally occurs in turkeys. Recently, we have cloned the LPDV provirus and established it as a replication-competent genome devoid of a viral oncogene [Gak et al., J. Virol. 63 (1989) 2877-2880]. This report presents the nucleotide sequence of its long terminal repeat (LTR) and establishes it as a potent transcriptional element. Several features of the LPDV LTR were similar to those found in the LTRs of the avian sarcoma-leukemia viruses (ASLV) and include the primer-binding site (tRNATrp), the polypurine tract, the organization of the polyadenylation signal, the complexities of the U3, R and U5 regions, as well as a potential secondary structure in U5-R. The LTR sequence diverges significantly from the ASLV LTRs, which share a common structure and have extensive sequence homology mainly in the R and U5 domains. These findings support the conclusion that LPDV represents a distinct class of avian retrovirus, evolutionarily related to the ASLV family.
AB - The lymphoproliferative disease virus (LPDV) is the etiological agent of a lymphoproliferative disease that naturally occurs in turkeys. Recently, we have cloned the LPDV provirus and established it as a replication-competent genome devoid of a viral oncogene [Gak et al., J. Virol. 63 (1989) 2877-2880]. This report presents the nucleotide sequence of its long terminal repeat (LTR) and establishes it as a potent transcriptional element. Several features of the LPDV LTR were similar to those found in the LTRs of the avian sarcoma-leukemia viruses (ASLV) and include the primer-binding site (tRNATrp), the polypurine tract, the organization of the polyadenylation signal, the complexities of the U3, R and U5 regions, as well as a potential secondary structure in U5-R. The LTR sequence diverges significantly from the ASLV LTRs, which share a common structure and have extensive sequence homology mainly in the R and U5 domains. These findings support the conclusion that LPDV represents a distinct class of avian retrovirus, evolutionarily related to the ASLV family.
KW - Recombinant DNA
KW - avian leukosis-sarcoma viruses
KW - chloramphenicol acetyltransferase
KW - retroviruses
UR - http://www.scopus.com/inward/record.url?scp=0025760966&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(91)90122-R
DO - 10.1016/0378-1119(91)90122-R
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0025760966
SN - 0378-1119
VL - 99
SP - 157
EP - 162
JO - Gene
JF - Gene
IS - 2
ER -
