TY - JOUR
T1 - Lymphoid cell infiltration into Epstein-Barr virus-positive nasopharyngeal carcinomas
AU - Shpitzer, Tommy
AU - Kerrebijn, Jeroen D.F.
AU - Freeman, Jeremy L.
AU - Hartwick, Warren
AU - Saiki, Yuriko
AU - Irish, Jonathan C.
AU - Macmillan, Christina M.
AU - Dosch, Hans Michael
N1 - Funding Information:
Supported by the Saul A. Silverman Family Foundation and Temmy Latner/Dynacare as a Canada International Scientific Program in Otolaryngology.
PY - 2001
Y1 - 2001
N2 - OBJECTIVES: A pilot study was designed fo analyze lymphoid cell infiltration in Epstein-Barr virus-positive (EBV+) nasopharyngeal carcinomas (NPCs) and to determine whether this pattern of infiltration is consistent' with non-EBV+ head and neck carcinomas or with solid EBV+ tumors in other locations. STUDY DESIGN: We performed a retrospective analysis of archived NPCs and oral cavity carcinomas. METHODS: Immunohistochemical staining of the archive material for various markers (CD3, CD8, UCHL-1, S-100, and intercellular adhesion molecule) was performed. Polymerase chain reaction techniques to establish the presence of the EBV genome were used. Cells in different locations were counted under a light microscope by 2 of the authors. RESULTS: The infiltration pattern of HPCs was different from that of oral cavity carcinomas. Stromal infiltration was significantly denser in oral cavity carcinomas. Tumor nest infiltratlon was more pronounced in NPCs. The pattern of infiltration was comparable with what has been described for other solid EBV+ tumors. CONCLUSIONS: The immune response to NPCs is likely to be strongly influenced by the presence of the EBV genome. The pattern of infiltration is similar to that of other non-head and neck EBV+ solid tumors and different from that of EBV- head and neck carcinomas.
AB - OBJECTIVES: A pilot study was designed fo analyze lymphoid cell infiltration in Epstein-Barr virus-positive (EBV+) nasopharyngeal carcinomas (NPCs) and to determine whether this pattern of infiltration is consistent' with non-EBV+ head and neck carcinomas or with solid EBV+ tumors in other locations. STUDY DESIGN: We performed a retrospective analysis of archived NPCs and oral cavity carcinomas. METHODS: Immunohistochemical staining of the archive material for various markers (CD3, CD8, UCHL-1, S-100, and intercellular adhesion molecule) was performed. Polymerase chain reaction techniques to establish the presence of the EBV genome were used. Cells in different locations were counted under a light microscope by 2 of the authors. RESULTS: The infiltration pattern of HPCs was different from that of oral cavity carcinomas. Stromal infiltration was significantly denser in oral cavity carcinomas. Tumor nest infiltratlon was more pronounced in NPCs. The pattern of infiltration was comparable with what has been described for other solid EBV+ tumors. CONCLUSIONS: The immune response to NPCs is likely to be strongly influenced by the presence of the EBV genome. The pattern of infiltration is similar to that of other non-head and neck EBV+ solid tumors and different from that of EBV- head and neck carcinomas.
UR - http://www.scopus.com/inward/record.url?scp=0035113816&partnerID=8YFLogxK
U2 - 10.1067/mhn.2001.111711
DO - 10.1067/mhn.2001.111711
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AN - SCOPUS:0035113816
SN - 0194-5998
VL - 124
SP - 188
EP - 194
JO - Otolaryngology - Head and Neck Surgery
JF - Otolaryngology - Head and Neck Surgery
IS - 2
ER -
