TY - JOUR
T1 - LYMPHOCYTE RESPONSE IN SARCOIDOSIS
AU - Topilsky, Marcel
AU - Williams, Marsha
AU - Siltzbach, Louis E.
AU - Glade, Philip R.
N1 - Funding Information:
We thank Dr. William Waithe and Dr. Photini Papageorgiou for helpful advice. This research was supported in part by research grant HE-13853-12Al from the National Heart and Lung Institute, research contract NIH 69-2078 within the Special Virus Cancer Program of the National Cancer Institute, and research grant RO1-All-0422 from the National Institutes of Allergy and Infectious Diseases, National Institutes of Health, U.S. Public Health Service. P. R. G. is a recipient of a research career development award (Al-46371) of the U.S. Public Health Service.
PY - 1972/1/15
Y1 - 1972/1/15
N2 - The response of peripheral lymphocytes of patients with Kveim positive sarcoidosis to a Chase-Siltzbach type-I (spleen J) suspension, normal spleen suspension, and phytohæmagglutinin (P.H.A.) was assayed in-vitro by 14C-leucine incorporation (nineteen patients), release of migration inhibitory factor (M.I.F.) (seventeen patients), and direct migration inhibition (six patients). No specific response to Kveim suspension in a wide range of doses was detected by these assays. All patients showed diminished responsiveness to P.H.A. when compared with controls. A high spontaneous release in vitro of M.I.F. was found for the lymphocytes of patients with sarcoidosis and could not be augmented by addition of Kveim antigen.
AB - The response of peripheral lymphocytes of patients with Kveim positive sarcoidosis to a Chase-Siltzbach type-I (spleen J) suspension, normal spleen suspension, and phytohæmagglutinin (P.H.A.) was assayed in-vitro by 14C-leucine incorporation (nineteen patients), release of migration inhibitory factor (M.I.F.) (seventeen patients), and direct migration inhibition (six patients). No specific response to Kveim suspension in a wide range of doses was detected by these assays. All patients showed diminished responsiveness to P.H.A. when compared with controls. A high spontaneous release in vitro of M.I.F. was found for the lymphocytes of patients with sarcoidosis and could not be augmented by addition of Kveim antigen.
UR - http://www.scopus.com/inward/record.url?scp=0015507064&partnerID=8YFLogxK
U2 - 10.1016/S0140-6736(72)90680-0
DO - 10.1016/S0140-6736(72)90680-0
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AN - SCOPUS:0015507064
VL - 299
SP - 117
EP - 120
JO - The Lancet
JF - The Lancet
SN - 0140-6736
IS - 7742
ER -