Lymphocyte activation by monovalent fragments of antibodies reactive with cell surface carbohydrates*

Ben Ami Sela, John L. Wang, Gerald M. Edelman

Research output: Contribution to journalArticlepeer-review

Abstract

Antibodies reactive with cell surface carbohydrates were isolated from normal chicken serum and were found to be mitogenic for mouse splenic lymphocytes as assayed by both blast transformation and [3H]thymidine incorporation. The Fab’ fragments of these carbohydrate-binding immunoglobulins were just as mitogenic as the divalent native antibody. Moreover, succinylated Fab’ fragments, which probably would not form self-associating aggregates, showed similar mitogenic properties. All of these results indicate that, at least for saccharide-specific ligands, multipoint attachment and receptor cross-linkage on the cell to which the ligand is attached may not be a stringent requirement for activation.

Original languageEnglish
Pages (from-to)665-671
Number of pages7
JournalJournal of Experimental Medicine
Volume143
Issue number3
DOIs
StatePublished - 1 Mar 1976
Externally publishedYes

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