@article{54c1d747ab9740deb810686a7c4623da,
title = "Luminescent Amphiphilic Aminoglycoside Probes to Study Transfection",
abstract = "We report the characterization of amphiphilic aminoglycoside conjugates containing luminophores with aggregation-induced emission properties as transfection reagents. These inherently luminescent transfection vectors are capable of binding plasmid DNA through electrostatic interactions; this binding results in an emission “on” signal due to restriction of intramolecular motion of the luminophore core. The luminescent cationic amphiphiles effectively transferred plasmid DNA into mammalian cells (HeLa, HEK 293T), as proven by expression of a red fluorescent protein marker. The morphologies of the aggregates were investigated by microscopy as well as ζ-potential and dynamic light-scattering measurements. The transfection efficiencies using luminescent cationic amphiphiles were similar to that of the gold-standard transfection reagent Lipofectamine{\textregistered} 2000.",
keywords = "aggregation-induced emission, bioimaging, cationic amphiphiles, self-assembly, transfection agents",
author = "Alexander Zimmermann and Jaber, {Qais Z.} and Johannes Koch and Steffen Riebe and Cecilia Vallet and Kateryna Loza and Matthias Hayduk and Steinbuch, {Kfir B.} and Knauer, {Shirley K.} and Micha Fridman and Jens Voskuhl",
note = "Publisher Copyright: {\textcopyright} 2021 The Authors. ChemBioChem published by Wiley-VCH GmbH",
year = "2021",
month = may,
day = "4",
doi = "10.1002/cbic.202000725",
language = "אנגלית",
volume = "22",
pages = "1563--1567",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "John Wiley and Sons Inc.",
number = "9",
}