Low-level light therapy induces mucosal healing in a murine model of dextran-sodium-sulfate induced colitis

Ehud Zigmond*, Chen Varol, Michail Kaplan, Oz Shapira, Ehud Melzer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Objective: The aim of this study was to demonstrate the effect of low-level light therapy (LLLT) in an acute colitis model in mice. Background data: Low-level light therapy (LLLT) has been shown to be an effective treatment for various inflammatory processes such as oral mucositis and diabetic foot ulcers. Methods: Colitis was induced by dextran sodium sulfate (DSS) in mice in four blinded controlled studies (validation of model, efficacy study, and two studies for evaluation of optimal dose). LLLT was applied to the colon utilizing a small diameter endoscope with an LED-based light source in several wavelengths (440, 660, and 850 nm at 1 J/cm2) and then 850 nm at several doses (1, 0.5, 0.25, and 0.1 J/cm2). LLLT was initiated 1 day prior to induction of colitis and went on for the 6 day induction period as well as for the following 3-10 days. Dose was controlled by changing exposure time. Disease activity was scored endoscopically and by histopathological assessment. Results: Statistically significant improvement in disease severity was observed in the treatment groups compared with the control groups. The three wavelengths used demonstrated efficacy, and a clear dose-response curve was observed for one of the wavelengths (850 nm). On day 11, colonoscopic scoring in the sham-treated mice increased from 7.9±1.3 to 12.2±2.2, while activity in all treated groups remained stable. Conclusions: Photobiostimulation with LLLT has a significant positive effect on disease progression in mice with DSS colitis.

Original languageEnglish
Pages (from-to)450-457
Number of pages8
JournalPhotomedicine and Laser Surgery
Issue number8
StatePublished - 1 Aug 2014


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