Low-intensity ultrasound induces angiogenesis in rat hind-limb ischemia

Sharon Barzelai; Orna Sharabani-Yosef; Radka Holbova; David Castel; Raphael Walden; Santiago Engelberg; Mickey Scheinowitz

doi:10.1016/j.ultrasmedbio.2005.08.010

Low-intensity ultrasound induces angiogenesis in rat hind-limb ischemia

Sharon Barzelai, Orna Sharabani-Yosef, Radka Holbova, David Castel, Raphael Walden, Santiago Engelberg, Mickey Scheinowitz^*

^*Corresponding author for this work

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

We investigated the effect of low-intensity ultrasound (US) on tissue blood flow and angiogenesis after limb ischemia in vivo. Rats underwent surgical ligation of the femoral or the iliac arteries. Half the animals were exposed to low-intensity US (0.05 W/cm²) during three consecutive sessions. At 3 weeks postsurgery, limb perfusion was assessed using laser Doppler and angiography. Immunostaining and vascular endothelial growth factor (VEGF) messenger ribonucleic acid (mRNA) expression were performed 7 d postsurgery. US irradiation significantly improved limb perfusion in both ischemic models (p = 0.04). Angiography showed increased blood vessels in the moderate ischemia (p = 0.01), but not in the severe ischemia (p = 0.19). Histology demonstrated a significantly higher number of blood vessels and proliferating cells in US-irradiated moderate and severe ischemia (p = 0.002 and p = 0.03, respectively). VEGF mRNA was significantly higher in moderate ischemia (p = 0.02). No differences in apoptotic cell death were evident in the models. Low-intensity US significantly improved tissue blood flow and angiogenesis, irrespective of the extent of the ischemia.

Original language	English
Pages (from-to)	139-145
Number of pages	7
Journal	Ultrasound in Medicine and Biology
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/j.ultrasmedbio.2005.08.010
State	Published - Jan 2006

Funding

Funders	Funder number
Tel Aviv University
Ministry of Science and Technology, Israel

Keywords

Apoptosis
Cell proliferation
Immunostaining
VEGF
mRNA

UN SDGs

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10.1016/j.ultrasmedbio.2005.08.010

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title = "Low-intensity ultrasound induces angiogenesis in rat hind-limb ischemia",

abstract = "We investigated the effect of low-intensity ultrasound (US) on tissue blood flow and angiogenesis after limb ischemia in vivo. Rats underwent surgical ligation of the femoral or the iliac arteries. Half the animals were exposed to low-intensity US (0.05 W/cm2) during three consecutive sessions. At 3 weeks postsurgery, limb perfusion was assessed using laser Doppler and angiography. Immunostaining and vascular endothelial growth factor (VEGF) messenger ribonucleic acid (mRNA) expression were performed 7 d postsurgery. US irradiation significantly improved limb perfusion in both ischemic models (p = 0.04). Angiography showed increased blood vessels in the moderate ischemia (p = 0.01), but not in the severe ischemia (p = 0.19). Histology demonstrated a significantly higher number of blood vessels and proliferating cells in US-irradiated moderate and severe ischemia (p = 0.002 and p = 0.03, respectively). VEGF mRNA was significantly higher in moderate ischemia (p = 0.02). No differences in apoptotic cell death were evident in the models. Low-intensity US significantly improved tissue blood flow and angiogenesis, irrespective of the extent of the ischemia.",

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note = "Funding Information: This work was performed as partial fulfillment for M.S. degree of Sharon Barzelai, Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, and was supported in part by the interdisciplinary grant, Israel Ministry of Science.",

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AU - Walden, Raphael

AU - Engelberg, Santiago

AU - Scheinowitz, Mickey

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Low-intensity ultrasound induces angiogenesis in rat hind-limb ischemia

Abstract

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Keywords

UN SDGs

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