TY - GEN
T1 - Low energy laser irradiation of stem cells for cardiac repair after myocardial infarction
AU - Tuby, H.
AU - Maltz, L.
AU - Oron, U.
PY - 2008
Y1 - 2008
N2 - The aim of the present study was to evaluate the possible beneficial effects of implantation of laser-irradiated mesenchymal stem cells (MSCs) into the infarcted rat heart. MSCs were isolated from rat bone marrow and grown in culture. The cells were laser irradiated with Ga-Al-As laser (810 nm wavelength), labeled with 5-Bromo-2'deoxyuridine (BrdU), and then implanted into infarcted rat hearts. Non-irradiated cells were similarly labeled and acted as control. Hearts were excised three weeks later and cells were stained for BrdU and c-kit (Stem cell marker) immunoreactivity. Infarcted hearts that were implanted with laser-treated cells showed a significant reduction of 53% in infarct size and a 5-6.3-fold significant increase in cell density that reacted positively to c-kit. The findings of the present study provide the first evidence that LLLT can significantly increase survival and/or proliferation of MSCs post implantation into the ischemic/infarcted heart, followed by a marked reduction of scarring, and enhanced angiogenesis.
AB - The aim of the present study was to evaluate the possible beneficial effects of implantation of laser-irradiated mesenchymal stem cells (MSCs) into the infarcted rat heart. MSCs were isolated from rat bone marrow and grown in culture. The cells were laser irradiated with Ga-Al-As laser (810 nm wavelength), labeled with 5-Bromo-2'deoxyuridine (BrdU), and then implanted into infarcted rat hearts. Non-irradiated cells were similarly labeled and acted as control. Hearts were excised three weeks later and cells were stained for BrdU and c-kit (Stem cell marker) immunoreactivity. Infarcted hearts that were implanted with laser-treated cells showed a significant reduction of 53% in infarct size and a 5-6.3-fold significant increase in cell density that reacted positively to c-kit. The findings of the present study provide the first evidence that LLLT can significantly increase survival and/or proliferation of MSCs post implantation into the ischemic/infarcted heart, followed by a marked reduction of scarring, and enhanced angiogenesis.
UR - http://www.scopus.com/inward/record.url?scp=84904099603&partnerID=8YFLogxK
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AN - SCOPUS:84904099603
SN - 9788875874704
T3 - Proceedings of the International Conference of the World Association of Laser Therapy, WALT 2008
SP - 91
EP - 96
BT - Proceedings of the International Conference of the World Association of Laser Therapy, WALT 2008
PB - MEDIMOND s.r.l.
T2 - 7th International Congress of the World Association of Laser Therapy, WALT 2008
Y2 - 19 October 2008 through 22 October 2008
ER -