Low affinity of the receptor for 1α, 25-dihydroxyvitamin D3 in the marmoset, a new world monkey

Uri A. Liberman, Donald de Grange, Stephen J. Marx

Research output: Contribution to journalArticlepeer-review

Abstract

Circulating levels of 1,25-dihydroxyvitamin D are 10-fold higher in the marmoset, a New World monkey, than in man; to assess hormone receptors, we evaluated interactions of 1,25-dihydroxy vitamin D3 with virus-transformed lympocytes. soluble extracts of transformed lymphocytes from humans showed hormone binding with affinity and capacity similar to that of receptors for 1,25-dihydroxyvitamin D from other human tissues. However, soluble extracts of transformed lymphocytes from the marmoset showed a strikingly lower affinity for 1,25-dihydroxyvitamin D (Kd 2.2 vs 0.27 nM in marmoset vs human) and a mildly lower binding capacity (6.9 vs 16 fmol mg protein). A defective receptor for 1,25-dihydroxyvitamin D3 could account for resistance of target tissues to this hormone in the marmoset.

Original languageEnglish
Pages (from-to)385-388
Number of pages4
JournalFEBS Letters
Volume182
Issue number2
DOIs
StatePublished - 25 Mar 1985
Externally publishedYes

Keywords

  • Hormone resistance
  • Lymphocyte
  • Osteomalacia
  • Receptor
  • Vitamin D

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