Loss and regain of virulence of a Candida albicans mutant as assessed in systemic murine candidiasis

A. Z. Roth-Ben, Z. Altboum, Y. Shadkchan, E. Segal

Research output: Contribution to journalArticlepeer-review

Abstract

Aim. The virulence ability of Candida albicans strain CBS562, its histidine auxotroph mutant (SAG5) and a respiratory-deficient mutant of the his-strain (SAR1) was tested in a murine model of systemic candidiasis. Results. The results demonstrated that both mutant strains were significantly less virulent in this animal model as compared to the wild type. This was demonstrated by decreased percentage of mortality, increased survival time and lower fungal load in target organs. To verify whether the prototrophy will restore the virulence ability, we tested the virulence ability of the histidine auxotroph transformant in comparison to that of the wild type, the his- mutant (SAG5) and the plasmid-cured transformant. The virulence of the his-transformant was similar to that of the wild type, whereas the virulence ability of the plasmid-cured strain was similar to that of SAG5. Conclusion. In summary, this study demonstrated loss of virulence in C. albicans mutants and regain of the virulence by transformation from auxotrophy to prototrophy.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalJournal de Mycologie Medicale
Volume11
Issue number3
StatePublished - 2001

Keywords

  • Candida albicans
  • Murine Candidiasis
  • Mutant
  • Virulence

Fingerprint

Dive into the research topics of 'Loss and regain of virulence of a Candida albicans mutant as assessed in systemic murine candidiasis'. Together they form a unique fingerprint.

Cite this