Locomotion through three-dimentional type I rat tail collagen. A modified mini-assay

H. Gutman; D. Risin; B. P. Katz; N. R. Pellis

doi:10.1016/0022-1759(93)90084-K

Locomotion through three-dimentional type I rat tail collagen. A modified mini-assay

H. Gutman, D. Risin, B. P. Katz, N. R. Pellis

Research output: Contribution to journal › Article › peer-review

Abstract

There is an increased application of three-dimensional type I rat tail collagen as an in vitro model for the peritumoral matrix in analysis of lymphocyte migration. The increased demand prompted us to modify the previous methods. We here describe our 'mini'-setup of the collagen model assay, which uses only 1 20 the amount of collagen medium and the number of cells used in the conventional assay. The modified assay was tested for optimal collagen concentration in gel for upward and downward migration, for locomotion from a collagen-gel bead into a collagen overlayer for demonstration of the effect of inhibitors and for differentiation between locomotory properties of lymphocyte subpopulations. The results verify that the mini-assay is an applicable in vitro model, easily read and amenable to limited blood samples such as those obtained from cancer patients, and reflects well known in vivo events.

Original language	English
Pages (from-to)	175-180
Number of pages	6
Journal	Journal of Immunological Methods
Volume	157
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(93)90084-K
State	Published - 4 Jan 1993
Externally published	Yes

Keywords

Interstitium
Locomotion
Locomotion inhibitor
Lymphocyte
Protease
Tumor matrix
Type I collagen

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0022-1759(93)90084-K

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title = "Locomotion through three-dimentional type I rat tail collagen. A modified mini-assay",

abstract = "There is an increased application of three-dimensional type I rat tail collagen as an in vitro model for the peritumoral matrix in analysis of lymphocyte migration. The increased demand prompted us to modify the previous methods. We here describe our 'mini'-setup of the collagen model assay, which uses only 1 20 the amount of collagen medium and the number of cells used in the conventional assay. The modified assay was tested for optimal collagen concentration in gel for upward and downward migration, for locomotion from a collagen-gel bead into a collagen overlayer for demonstration of the effect of inhibitors and for differentiation between locomotory properties of lymphocyte subpopulations. The results verify that the mini-assay is an applicable in vitro model, easily read and amenable to limited blood samples such as those obtained from cancer patients, and reflects well known in vivo events.",

keywords = "Interstitium, Locomotion, Locomotion inhibitor, Lymphocyte, Protease, Tumor matrix, Type I collagen",

author = "H. Gutman and D. Risin and Katz, {B. P.} and Pellis, {N. R.}",

note = "Funding Information: This work was supported by the Gillson Lon-genbaugh Foundation, National Institutes of Health Biological Research Support Grant RR5511, Texas Coordinating Board Advanced Technology Program grant 000015-131, and National Cancer Institute training grant CA-09599. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Cancer Institute. H. Gutman is supported by the Israel Cancer Association, the American Physician Fellowship for Medicine in Israel, and the Weissman Scholarship awarded by the Dean of the Sackler School of Medicine, Tel Aviv University, Israel.",

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N1 - Funding Information: This work was supported by the Gillson Lon-genbaugh Foundation, National Institutes of Health Biological Research Support Grant RR5511, Texas Coordinating Board Advanced Technology Program grant 000015-131, and National Cancer Institute training grant CA-09599. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Cancer Institute. H. Gutman is supported by the Israel Cancer Association, the American Physician Fellowship for Medicine in Israel, and the Weissman Scholarship awarded by the Dean of the Sackler School of Medicine, Tel Aviv University, Israel.

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AB - There is an increased application of three-dimensional type I rat tail collagen as an in vitro model for the peritumoral matrix in analysis of lymphocyte migration. The increased demand prompted us to modify the previous methods. We here describe our 'mini'-setup of the collagen model assay, which uses only 1 20 the amount of collagen medium and the number of cells used in the conventional assay. The modified assay was tested for optimal collagen concentration in gel for upward and downward migration, for locomotion from a collagen-gel bead into a collagen overlayer for demonstration of the effect of inhibitors and for differentiation between locomotory properties of lymphocyte subpopulations. The results verify that the mini-assay is an applicable in vitro model, easily read and amenable to limited blood samples such as those obtained from cancer patients, and reflects well known in vivo events.

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KW - Locomotion inhibitor

KW - Lymphocyte

KW - Protease

KW - Tumor matrix

KW - Type I collagen

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Locomotion through three-dimentional type I rat tail collagen. A modified mini-assay

Abstract

Keywords

UN SDGs

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