Localization of glycine neurotransmitter transporter (GLYT2) reveals correlation with the distribution of glycine receptor

Frantisek Jursky, Nathan Nelson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

132 Scopus citations

Abstract

We studied by immunocytochemical localization, the glycine neurotransmitter transporter (GLYT2) in mouse brain, using polyclonal antibodies raised against recombinant N-terminus and loop fusion proteins. Western analysis and immunocytochemistry of mouse brain frozen sections revealed caudal-rostral gradient of GLYT2 distribution with massive accumulation in the spinal cord, brainstem, and less in the cerebellum. Immunoreactivity was detected in processes with varicosities but not cell bodies. A correlation was observed between the pattern we obtained and previously reported strychnine binding studies. The results indicate that GLYT2 is involved in the termination of glycine neurotransmission accompanying the glycine receptor at the classic inhibitory system in the hindbrain.

Original languageEnglish
Pages (from-to)1026-1033
Number of pages8
JournalJournal of Neurochemistry
Volume64
Issue number3
StatePublished - Mar 1995
Externally publishedYes

Keywords

  • Glycine
  • Immunocytochemistry
  • Neurotransmitter
  • Transporter

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