Local penetration of gentamicin into the femoral cortex of rats

Y. Mirovsky*, N. Halperin, L. Copeliovitch

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


To investigate the extent of local penetration of antibiotic into the bone, a single dose of 10 mg/kg gentamicin sulphate was injected over the midshaft of the left femur in 12 young and 14 old rats. In some of the rats the injected gentamicin was dissolved in saline while in the others it was dissolved in dimethylsulphoxide (DMSO). Up to 120 minutes after the injection gentamicin concentrations were measured by the fluoroimmunoassay method in the serum, cortex and spongiosa of both femors. In young rats gentamicin was shown to have penetrated directly into the bone and its presence was limited to the femoral cortex. In the old rats, on the other hand hematogenic was found to be the antibiotic spread in the femors. No significant difference was observed between DMSO and saline as solvents in facilitating gentamicin penetration into the bone or capillaries in either age group.

Original languageEnglish
Pages (from-to)238-241
Number of pages4
JournalArchives of Orthopaedic and Traumatic Surgery
Issue number4
StatePublished - Feb 1984
Externally publishedYes


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