TY - JOUR
T1 - Loading Capacity versus Enzyme Activity in Anisotropic and Spherical Calcium Carbonate Microparticles
AU - Donatan, Senem
AU - Yashchenok, Alexey
AU - Khan, Nazimuddin
AU - Parakhonskiy, Bogdan
AU - Cocquyt, Melissa
AU - Pinchasik, Bat El
AU - Khalenkow, Dmitry
AU - Möhwald, Helmuth
AU - Konrad, Manfred
AU - Skirtach, Andre
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/6/8
Y1 - 2016/6/8
N2 - A new method of fabrication of calcium carbonate microparticles of ellipsoidal, rhomboidal, and spherical geometries is reported by adjusting the relative concentration ratios of the initial salt solutions and/or the ethylene glycol content in the reaction medium. Morphology, porosity, crystallinity, and loading capacity of synthesized CaCO3 templates were characterized in detail. Particles harboring dextran or the enzyme guanylate kinase were obtained through encapsulation of these macromolecules using the layer-by-layer assembly technique to deposit positively and negatively charged polymers on these differently shaped CaCO3 templates and were characterized by confocal laser scanning fluorescence microscopy, fluorometric techniques, and enzyme activity measurements. The enzymatic activity, an important application of such porous particles and containers, has been analyzed in comparison with the loading capacity and geometry. Our results reveal that the particles' shape influences morphology of particles and that, as a result, affects the activity of the encapsulated enzymes, in addition to the earlier reported influence on cellular uptake. These particles are promising candidates for efficient drug delivery due to their relatively high loading capacity, biocompatibility, and easy fabrication and handling.
AB - A new method of fabrication of calcium carbonate microparticles of ellipsoidal, rhomboidal, and spherical geometries is reported by adjusting the relative concentration ratios of the initial salt solutions and/or the ethylene glycol content in the reaction medium. Morphology, porosity, crystallinity, and loading capacity of synthesized CaCO3 templates were characterized in detail. Particles harboring dextran or the enzyme guanylate kinase were obtained through encapsulation of these macromolecules using the layer-by-layer assembly technique to deposit positively and negatively charged polymers on these differently shaped CaCO3 templates and were characterized by confocal laser scanning fluorescence microscopy, fluorometric techniques, and enzyme activity measurements. The enzymatic activity, an important application of such porous particles and containers, has been analyzed in comparison with the loading capacity and geometry. Our results reveal that the particles' shape influences morphology of particles and that, as a result, affects the activity of the encapsulated enzymes, in addition to the earlier reported influence on cellular uptake. These particles are promising candidates for efficient drug delivery due to their relatively high loading capacity, biocompatibility, and easy fabrication and handling.
KW - calcium carbonate
KW - enzyme
KW - enzyme-catalyzed reaction
KW - polyelectrolyte
KW - vaterite
UR - http://www.scopus.com/inward/record.url?scp=84973638658&partnerID=8YFLogxK
U2 - 10.1021/acsami.6b03492
DO - 10.1021/acsami.6b03492
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C2 - 27166641
AN - SCOPUS:84973638658
SN - 1944-8244
VL - 8
SP - 14284
EP - 14292
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 22
ER -