TY - JOUR
T1 - Living Bacteria in Thermoresponsive Gel for Treating Fungal Infections
AU - Lufton, Maayan
AU - Bustan, Or
AU - Eylon, Bat hen
AU - Shtifman-Segal, Ella
AU - Croitoru-Sadger, Tsuf
AU - Shagan, Alona
AU - Shabtay-Orbach, Ayelet
AU - Corem-Salkmon, Enav
AU - Berman, Judith
AU - Nyska, Abraham
AU - Mizrahi, Boaz
N1 - Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/10/4
Y1 - 2018/10/4
N2 - The leading living bacteria formulations currently available are from a limited list of genera and are generally limited to gastrointestinal tract syndromes. A formulation composed of living Bacillus subtilis incorporated in a thermoresponsive hydrogel that hardens after administration on the skin and continuously produces antifungal agents is described. The ability of the formula to support bacteria growth and its mechanical properties and penetrability through the skin are fine-tuned by varying the ratio between polymer concentrations and bacterial media. The formula penetrates via the stratum corneum and accumulates in the epidermis without penetrating the inner, dermis layer. In vivo results mirror the results seen in vitro: bacillus formulations completely inhibit candida growth, demonstrating clinical effects comparable to those achieved by ketoconazole. LC-MS/MS analysis of the bacterial formulation confirms the presence of surfactin, the most powerful biosurfactant that possesses a broad antifungal activity. This platform may enable rational design of novel formulations composed of secreting bacteria inside a responsive, smart, hydrogel—which is the prerequisite for producing a successful drug delivery system.
AB - The leading living bacteria formulations currently available are from a limited list of genera and are generally limited to gastrointestinal tract syndromes. A formulation composed of living Bacillus subtilis incorporated in a thermoresponsive hydrogel that hardens after administration on the skin and continuously produces antifungal agents is described. The ability of the formula to support bacteria growth and its mechanical properties and penetrability through the skin are fine-tuned by varying the ratio between polymer concentrations and bacterial media. The formula penetrates via the stratum corneum and accumulates in the epidermis without penetrating the inner, dermis layer. In vivo results mirror the results seen in vitro: bacillus formulations completely inhibit candida growth, demonstrating clinical effects comparable to those achieved by ketoconazole. LC-MS/MS analysis of the bacterial formulation confirms the presence of surfactin, the most powerful biosurfactant that possesses a broad antifungal activity. This platform may enable rational design of novel formulations composed of secreting bacteria inside a responsive, smart, hydrogel—which is the prerequisite for producing a successful drug delivery system.
KW - bacteria
KW - drug delivery
KW - fungal infections
KW - hydrogels
KW - stimuli responsive hydrogels
UR - http://www.scopus.com/inward/record.url?scp=85052403905&partnerID=8YFLogxK
U2 - 10.1002/adfm.201801581
DO - 10.1002/adfm.201801581
M3 - מאמר
AN - SCOPUS:85052403905
VL - 28
JO - Advanced Functional Materials
JF - Advanced Functional Materials
SN - 1616-301X
IS - 40
M1 - 1801581
ER -