Linear One-Dimensional Assembly of Metal Nanostructures onto an Asymmetric Peptide Nanofiber with High Persistence Length

Sudipta Mondal*, Pavel Rehak, Nandita Ghosh, Petr Král, Ehud Gazit*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Self-assembled peptide fibrils have been used extensively to template the organization of metal nanoparticles in a one-dimensional (1D) array. It has been observed that the formation of the 1D arrays with a width of a single or few nanoparticles (viz. 20 nm diameter) is only possible if the templating fibers have comparable diameters (viz. ≤20 nm). Accordingly, until today, all the peptide-based templates enabling such 1D arrays have very low persistence lengths, a property that depends strongly on the diameter of the template, owing to the inherent flexibility of only a few nanometer-wide fibers. Here, we demonstrate the formation of high persistence length 1D arrays templated by a short self-assembling peptide fibril with an asymmetrically distributed charged surface. The asymmetric nature of the peptide fibril allows charge-dependent deposition of the nanoparticles only to the part of the fiber with complementary charges, and the rest of the fibril surface remains free of nanoparticles. Consequently, fibers with a much higher diameter, which will have a higher persistence length, are able to template single or few nanoparticle-wide 1D arrays. Detailed microscopy, molecular dynamics simulations, and crystal structure analysis provide molecular-level insights into fiber asymmetry and its interactions with diverse nanostructures such as gold and magnetic nanoparticles. This study will afford an alternative paradigm for high persistence length 1D array fabrication comparable to DNA nanotechnology and lithography but with tremendous cost-effectiveness.

Original languageEnglish
Pages (from-to)18307-18314
Number of pages8
JournalACS Nano
Volume16
Issue number11
DOIs
StatePublished - 22 Nov 2022

Funding

FundersFunder number
Horizon 2020 Framework Programme
European Research Council
Department of Science and Technology, Ministry of Science and Technology, IndiaSRG/2019/001058
Department of Science and Technology, Ministry of Science and Technology, India
Horizon 2020694426
Horizon 2020

    Keywords

    • asymmetric fibers
    • gold nanoparticles
    • magnetic nanoparticles
    • one-dimensional nanoparticle arrays
    • peptide self-assembly

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