TY - JOUR
T1 - Light-Controlled Selective Collection-and-Release of Biomolecules by an On-Chip Nanostructured Device
AU - Borberg, Ella
AU - Zverzhinetsky, Marina
AU - Krivitsky, Adva
AU - Kosloff, Alon
AU - Heifler, Omri
AU - Degabli, Gal
AU - Soroka, Hagit Peretz
AU - Fainaro, Ronit Satchi
AU - Burstein, Larisa
AU - Reuveni, Shlomi
AU - Diamant, Haim
AU - Krivitsky, Vadim
AU - Patolsky, Fernando
N1 - Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/9/11
Y1 - 2019/9/11
N2 - The analysis of biosamples, e.g., blood, is a ubiquitous task of proteomics, genomics, and biosensing fields; yet, it still faces multiple challenges, one of the greatest being the selective separation and detection of target proteins from these complex biosamples. Here, we demonstrate the development of an on-chip light-triggered reusable nanostructured selective and quantitative protein separation and preconcentration platform for the direct analysis of complex biosamples. The on-chip selective separation of required protein analytes from raw biosamples is performed using antibody-photoacid-modified Si nanopillars vertical arrays (SiNPs) of ultralarge binding surface area and enormously high binding affinity, followed by the light-controlled rapid release of the tightly bound target proteins in a controlled liquid media. Two important experimental observations are presented: (1) the first demonstration on the control of biological reaction binding affinity by the nanostructuring of the capturing surface, leading to highly efficient protein collection capabilities, and (2) the light-triggered switching of the highly sticky binding surfaces into highly reflective nonbinding surfaces, leading to the rapid and quantitative release of the originally tightly bound protein species. Both of these two novel behaviors were theoretically and experimentally investigated. Importantly, this is the first demonstration of a three-dimensional (3D) SiNPs on-chip filter with ultralarge binding surface area and reversible light-controlled quantitative release of adsorbed biomolecules for direct purification of blood samples, able to selectively collect and separate specific low abundant proteins, while easily removing unwanted blood components (proteins, cells) and achieving desalting results, without the requirement of time-consuming centrifugation steps, the use of desalting membranes, or affinity columns.
AB - The analysis of biosamples, e.g., blood, is a ubiquitous task of proteomics, genomics, and biosensing fields; yet, it still faces multiple challenges, one of the greatest being the selective separation and detection of target proteins from these complex biosamples. Here, we demonstrate the development of an on-chip light-triggered reusable nanostructured selective and quantitative protein separation and preconcentration platform for the direct analysis of complex biosamples. The on-chip selective separation of required protein analytes from raw biosamples is performed using antibody-photoacid-modified Si nanopillars vertical arrays (SiNPs) of ultralarge binding surface area and enormously high binding affinity, followed by the light-controlled rapid release of the tightly bound target proteins in a controlled liquid media. Two important experimental observations are presented: (1) the first demonstration on the control of biological reaction binding affinity by the nanostructuring of the capturing surface, leading to highly efficient protein collection capabilities, and (2) the light-triggered switching of the highly sticky binding surfaces into highly reflective nonbinding surfaces, leading to the rapid and quantitative release of the originally tightly bound protein species. Both of these two novel behaviors were theoretically and experimentally investigated. Importantly, this is the first demonstration of a three-dimensional (3D) SiNPs on-chip filter with ultralarge binding surface area and reversible light-controlled quantitative release of adsorbed biomolecules for direct purification of blood samples, able to selectively collect and separate specific low abundant proteins, while easily removing unwanted blood components (proteins, cells) and achieving desalting results, without the requirement of time-consuming centrifugation steps, the use of desalting membranes, or affinity columns.
KW - biomolecules
KW - filtering
KW - light control
KW - photoacid
KW - preconcentration
KW - silicon nanopillars
UR - http://www.scopus.com/inward/record.url?scp=85071697717&partnerID=8YFLogxK
U2 - 10.1021/acs.nanolett.9b01323
DO - 10.1021/acs.nanolett.9b01323
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AN - SCOPUS:85071697717
SN - 1530-6984
VL - 19
SP - 5868
EP - 5878
JO - Nano Letters
JF - Nano Letters
IS - 9
ER -