Objective: We aimed to develop a new technique for treatment of granulation tissue (GT) growth using local hyperthermia. Methods: A temperature-controlled diode laser system was developed for induction of mild hyperthermia in real time. GT was generated by harvesting the skin over the gluteal fascia in rats. Histopathological analysis was used to estimate the effect of hyperthermia on the tissue. Results: In untreated rats, GT was detected within 3 days and reached maximal thickness after 12 days. Hyperthermia at 43°C and above significantly decreased GT thickness (n = 8 per group). Hyperthermia at 48°C for 3 minutes was the most efficient parameter for treatment of GT (51% reduction), with minimal (5%) muscle necrosis. Conclusions: Hyperthermia can significantly inhibit GT growth, with minimal damage to surrounding structures. Our findings suggest a possible role for hyperthermia as a therapeutic model against GT. Further research and long-term studies are needed to explore the utility of laser-induced hyperthermia for inhibition of GT growth.