Laser activated fluorescence measurements and morphological features: An in vivo study of clearance time of fluorescein isothiocyanate tagged cell markers

I. Gannot*, G. Gannot, A. Garashi, A. Gandjbakhche, A. Buchner, Y. Keisari

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Fourteen BALB/c mice were divided into two groups. One group served as the control and the second group was injected with a squamous cell carcinoma cell line to the tongue. After tumor development (1-4 weeks), mice were injected with a FITC conjugated CD3 marker to their tongues. Immediately after the marker injection, the clearance of the marker was measured using a laser spectroscopy system. The markers were excited by an argon laser at 488 nm and the fluorescence signal was measured as a function of time. A biopsy was taken from every mouse after the procedure and the excised tissue was histologically evaluated. Analysis of clearance times revealed a second order exponential decay for both groups with a slower pace of signal clearance for the sick mice.

Original languageEnglish
Pages (from-to)14-19
Number of pages6
JournalJournal of Biomedical Optics
Volume7
Issue number1
DOIs
StatePublished - Jan 2002

Funding

FundersFunder number
Ministry of Science, Technology, Culture and Sport8445
Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentZ01HD000261
United States-Israel Binational Science Foundation1998308

    Keywords

    • Fluorescence
    • Lasers
    • Tissues

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