Large, but not small sustained tensile strains stimulate adipogenesis in culture

Ayelet Levy, Sarit Enzer, Naama Shoham, Uri Zaretsky, Amit Gefen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Understanding the mechanoresponsiveness of adipocytes and the characteristics of the mechanical stimuli that regulate adipogenesis is critically important in establishing knowledge in regard to the long-term effects of a sedentary lifestyle (or immobility in extreme medical conditions) as well as concerning obesity and related diseases. In this study we subjected 3T3-L1 preadipocytes cultured on elastic substrata to different levels of static equiaxial tensile strains within the physiological range, up to substrate tensile strain (STS) of 12%, while inducing differentiation in the cultures. Based on prior work which revealed that adipogenesis is accelerated in cultures subjected to STS of 12% by activating the mitogenactivated protein kinase kinase signaling pathway, we were specifically interested in identifying the STS levels which trigger this process. We hence monitored the production and accumulation of lipid droplets (LDs) using a non-destructive, image-processing-based method that we have previously developed, for a period of 4 weeks. The experimental data demonstrated accelerated adipogenesis in the cultures subjected to STS levels of 6%, 9%, and 12% with respect to cultures subjected to STS of 3% and (non-stretched) control cultures. This accelerated adipogenic response to the large sustained STS manifested in significantly larger numbers and greater sizes of LDs in the cultures that were stretched to large STS levels (p<0.05), starting at approximately day 14 following induction of differentiation. Hence, indeed, there appears to be a certain tensile strain threshold, or domain- which is found within the physiological range-above which the responsiveness of adipocytes to sustained static stretching increases and is manifested in accelerated adipogenesis.

Original languageEnglish
Pages (from-to)1052-1060
Number of pages9
JournalAnnals of Biomedical Engineering
Issue number5
StatePublished - May 2012


FundersFunder number
Ela Kodesz Institute for Cardiac Physical Sciences and Engineering
Ministry of Science and Technology, Taiwan


    • 3T3-L1 preadipocytes
    • Differentiation
    • Lipid droplets
    • Mechanotrasduction
    • Strecth deformation


    Dive into the research topics of 'Large, but not small sustained tensile strains stimulate adipogenesis in culture'. Together they form a unique fingerprint.

    Cite this