Lack of correlation between apoptosis and DNA single-strand breaks in X- irradiated human peripheral blood mononuclear cells in the course of ageing

Christian Chauvin, Evelyn Heidenreich, Kristina Elmendorff-Dreikorn, Hanoch Slor, Joachim Kutzner, Renato Batel, Heinz C. Schröder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The dependence on age of both the basal and the X-radiation-induced levels of apoptosis was examined in human peripheral blood mononuclear cells (PBMC). In the same samples, the base value and the extent of induced DNA single-strand breaks were determined, using a sensitive and fast microplate assay. PBMC were isolated from blood of donors of various age groups (20-30, 40-60 and > 70 years of age) and X-irradiated ex vivo using a 6 MV linear accelerator to give a total exposure of 4 Gy. The mean basal levels of apoptosis in PBMC from donors in the 40-60 year age group and the > 70 year age group were found to be only slightly higher (by 20-10%) compared to that of the 20-30 year age group, whereas the extent of DNA damage strongly and significantly (P<0.01) increased with age by up to 2-fold. In contrast to the extent of induced DNA damage, which steadily increased in the course of ageing by up to 1.8-fold, there was only a transient increase in the level of induced apoptosis to 1.5-fold in PBMC from X-irradiated blood (4 Gy photons) from donors aged 40-60 followed by a decrease to 0.9-fold in PBMC from old donors (> 70), compared to age group 20-30. The results show that X-ray- induced apoptosis and DNA damage in PBMC are not correlated during ageing.

Original languageEnglish
Pages (from-to)117-128
Number of pages12
JournalMechanisms of Ageing and Development
Volume106
Issue number1-2
DOIs
StatePublished - 1 Dec 1998

Keywords

  • Ageing
  • Apoptosis
  • DNA single-strand breaks
  • DNA unwinding
  • X-irradiation

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