TY - JOUR
T1 - l-Amino acid oxidase from Vipera palaestinae venom
T2 - Purification and assay
AU - Shaham, Nurit
AU - Bdolah, Avner
PY - 1973/12/15
Y1 - 1973/12/15
N2 - 1. 1. l-Amino acid oxidase was isolated from the venom of Vipera palaestinae by a procedure involving heat treatment, ammonium sulfate precipitation, gel filtration, adsorption on calcium phosphate gel and chromatography on DEAE-cellulose. 2. 2. The enzyme was homogeneous in the ultracentrifuge and had a molecular weight of approximately 130,000. The pure enzyme showed on acrylamide electrophoresis at least three bands with enzymic activity. 3. 3. l-Amino acid oxidase was assayed with l-leucine as a substrate and phenol-indo-2 ; 6-dichlorophenol as an electron acceptor. This assay was compared to another one using l-kynurenine as a substrate. 4. 4. Optimal conditions for storage of the enzyme were found to be either at -15°C with 0·2 M acetate or under nitrogen at 0°C.
AB - 1. 1. l-Amino acid oxidase was isolated from the venom of Vipera palaestinae by a procedure involving heat treatment, ammonium sulfate precipitation, gel filtration, adsorption on calcium phosphate gel and chromatography on DEAE-cellulose. 2. 2. The enzyme was homogeneous in the ultracentrifuge and had a molecular weight of approximately 130,000. The pure enzyme showed on acrylamide electrophoresis at least three bands with enzymic activity. 3. 3. l-Amino acid oxidase was assayed with l-leucine as a substrate and phenol-indo-2 ; 6-dichlorophenol as an electron acceptor. This assay was compared to another one using l-kynurenine as a substrate. 4. 4. Optimal conditions for storage of the enzyme were found to be either at -15°C with 0·2 M acetate or under nitrogen at 0°C.
KW - Vipera palaestinae
KW - l-Amino oxidase
KW - venom
UR - http://www.scopus.com/inward/record.url?scp=49549172908&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(73)90113-2
DO - 10.1016/0305-0491(73)90113-2
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AN - SCOPUS:49549172908
VL - 46
SP - 691-696,IN3-IN4,697-698
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 4
ER -