TY - JOUR
T1 - Junctional epidermolysis bullosa in the Middle East
T2 - Clinical and genetic studies in a series of consanguineous families
AU - Nakano, Aoi
AU - Lestringant, Gilles G.
AU - Paperna, Tamar
AU - Bergman, Reuven
AU - Gershoni, Ruth
AU - Frossard, Philippe
AU - Kanaan, Moien
AU - Meneguzzi, Guerrino
AU - Richard, Gabriele
AU - Pfendner, Ellen
AU - Uitto, Jouni
AU - Pulkkinen, Leena
AU - Sprecher, Eli
N1 - Funding Information:
We acknowledge the support of DebRA of the United States for the DebRA Molecular Diagnostics Laboratory at the Department of Dermatology and Cutaneous Biology of Thomas Jefferson University. This study was supported by the US Public Health Service, National Institutes of Health grant POI-AR38923. Dr Sprecher is supported by the American Physicians Fellowship for Medicine in Israel.
PY - 2002/4/1
Y1 - 2002/4/1
N2 - Background: Junctional epidermolysis bullosa (JEB) is a group of inherited blistering diseases characterized by epidermal-dermal separation resulting from mutations that affect the function of critical components of the basement membrane zone. This group of autosomal recessive diseases is especially prevalent in regions where consanguinity is common, such as the Middle East. However, the clinical and genetic epidemiology of JEB in this region remains largely unexplored. Objective: The aim of the present study was to assess a series of consanguineous JEB families originating from the Middle East. Methods: We identified 7 families referred to us between 1998 and 1999 and originating from the United Arab Emirates, Saudi Arabia, Sudan, Yemen, and Israel. Histologic, immunofluorescence, and electron microscopy studies were performed to direct the subsequent molecular analysis. DNA obtained from all family members was amplified by means of polymerase chain reaction and analyzed by conformation-sensitive gel electrophoresis with subsequent direct sequencing. Results: In 6 families presenting with the clinical and histologic features distinctive for JEB, mutations in genes encoding 1 of the 3 subunit polypeptides of laminin-5 were identified. Two families each had mutations in LAMB3, 2 in LAMA3, and 2 in LAMC2. Out of 7 distinct mutations, 5 were novel and 2 were recurrent. No relationship was found between the presence of nonsense/frameshift mutations in laminin-5 genes and perinatal mortality, contradicting a major genotype-phenotype correlation previously reported in the European and US literature. Similarly, none of the recurrent LAMB3 hot spot mutations previously described in other populations was found in our series. Finally, in a family with the clinical diagnosis of generalized atrophic benign epidermolysis bullosa, a homozygous non-sense mutation in Col17A1 gene (encoding the BPAG2 antigen) was identified. Conclusion: The present report suggests (1) the existence of a unique spectrum of mutations in the Middle East populations and (2) the need for the implementation of a diagnostic strategy tailored to the genetic features of JEB in this region.
AB - Background: Junctional epidermolysis bullosa (JEB) is a group of inherited blistering diseases characterized by epidermal-dermal separation resulting from mutations that affect the function of critical components of the basement membrane zone. This group of autosomal recessive diseases is especially prevalent in regions where consanguinity is common, such as the Middle East. However, the clinical and genetic epidemiology of JEB in this region remains largely unexplored. Objective: The aim of the present study was to assess a series of consanguineous JEB families originating from the Middle East. Methods: We identified 7 families referred to us between 1998 and 1999 and originating from the United Arab Emirates, Saudi Arabia, Sudan, Yemen, and Israel. Histologic, immunofluorescence, and electron microscopy studies were performed to direct the subsequent molecular analysis. DNA obtained from all family members was amplified by means of polymerase chain reaction and analyzed by conformation-sensitive gel electrophoresis with subsequent direct sequencing. Results: In 6 families presenting with the clinical and histologic features distinctive for JEB, mutations in genes encoding 1 of the 3 subunit polypeptides of laminin-5 were identified. Two families each had mutations in LAMB3, 2 in LAMA3, and 2 in LAMC2. Out of 7 distinct mutations, 5 were novel and 2 were recurrent. No relationship was found between the presence of nonsense/frameshift mutations in laminin-5 genes and perinatal mortality, contradicting a major genotype-phenotype correlation previously reported in the European and US literature. Similarly, none of the recurrent LAMB3 hot spot mutations previously described in other populations was found in our series. Finally, in a family with the clinical diagnosis of generalized atrophic benign epidermolysis bullosa, a homozygous non-sense mutation in Col17A1 gene (encoding the BPAG2 antigen) was identified. Conclusion: The present report suggests (1) the existence of a unique spectrum of mutations in the Middle East populations and (2) the need for the implementation of a diagnostic strategy tailored to the genetic features of JEB in this region.
UR - http://www.scopus.com/inward/record.url?scp=0036553471&partnerID=8YFLogxK
U2 - 10.1067/mjd.2002.119673
DO - 10.1067/mjd.2002.119673
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AN - SCOPUS:0036553471
SN - 0190-9622
VL - 46
SP - 510
EP - 516
JO - Journal of the American Academy of Dermatology
JF - Journal of the American Academy of Dermatology
IS - 4
ER -
