Jagged-1hi or Delta-1hi thymic stromal cell populations can trigger acquisition of Thy-1 and loss of B220

Myra Small*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Effects of endogenous Notch ligands Jagged-1 and Delta-1 on maturation markers in adult mouse lymphocytes were checked in vitro on putative thymocyte precursors from bone marrow and on DN3 cells from the thymus. Both populations were cocultured with stromal cells (Small M, Histochem Cell Biol 2005;123:513) naturally expressing high levels of either Jagged-1 or Delta-1. Initially the bone marrow cells were Thy-1.2neg while the majority were B220hi. These trends were reversed by culture on both the Jagged-1hi and Delta-1hi monolayers. Then DN3 thymocytes were cultured with stroma expressing these two ligands and the T cell receptor β chain was expressed more clearly after coculture on Delta-1hi stroma. These findings indicate that stromal cells with endogenous Jagged-1 as well as Delta-1 could initiate early changes toward T cell commitment while Delta-1 favored later αβ-TCR development.

Original languageEnglish
Pages (from-to)966-973
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume30
Issue number10
DOIs
StatePublished - 2006

Funding

FundersFunder number
Carl and Leonora Fingerhut fund of Tel Aviv University

    Keywords

    • Delta-1
    • Endogenous ligands
    • Jagged-1
    • Notch
    • Notch ligands
    • T cell precursors
    • Thymic stroma
    • Thymocyte development

    Fingerprint

    Dive into the research topics of 'Jagged-1hi or Delta-1hi thymic stromal cell populations can trigger acquisition of Thy-1 and loss of B220'. Together they form a unique fingerprint.

    Cite this