Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae

Amy Rosenbluh, M. Mevarech, Yigal Koltin, Jessica A. Gorman

Research output: Contribution to journalArticlepeer-review

Abstract

A genomic library of the asexual pathogenic yeast Candida albicans was constructed in the S. cerevisiae vector YEp13. The library contains a representation of the entire genome with a probability of 99%. The expression of the genes of C. albicans in S. cerevisiae was examined and two mutations his3-1 and trp1-289 of S. cerevisiae were complemented by the cloned genes of C. albicans. The hybridization data indicates that the plasmids complementing the mutations of S. cerevisiae contain sequences from C. albicans.

Original languageEnglish
Pages (from-to)500-502
Number of pages3
JournalMolecular Genetics and Genomics
Volume200
Issue number3
DOIs
StatePublished - Aug 1985

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