Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae

Amy Rosenbluh; M. Mevarech; Yigal Koltin; Jessica A. Gorman

doi:10.1007/BF00425739

Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae

Amy Rosenbluh^*, M. Mevarech, Yigal Koltin, Jessica A. Gorman

^*Corresponding author for this work

Biotechnology

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Abstract

A genomic library of the asexual pathogenic yeast Candida albicans was constructed in the S. cerevisiae vector YEp13. The library contains a representation of the entire genome with a probability of 99%. The expression of the genes of C. albicans in S. cerevisiae was examined and two mutations his3-1 and trp1-289 of S. cerevisiae were complemented by the cloned genes of C. albicans. The hybridization data indicates that the plasmids complementing the mutations of S. cerevisiae contain sequences from C. albicans.

Original language	English
Pages (from-to)	500-502
Number of pages	3
Journal	Molecular Genetics and Genomics
Volume	200
Issue number	3
DOIs	https://doi.org/10.1007/BF00425739
State	Published - Aug 1985

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AB - A genomic library of the asexual pathogenic yeast Candida albicans was constructed in the S. cerevisiae vector YEp13. The library contains a representation of the entire genome with a probability of 99%. The expression of the genes of C. albicans in S. cerevisiae was examined and two mutations his3-1 and trp1-289 of S. cerevisiae were complemented by the cloned genes of C. albicans. The hybridization data indicates that the plasmids complementing the mutations of S. cerevisiae contain sequences from C. albicans.

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Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae

Abstract

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