Isolation of extracellular RNA from serum/plasma

Justyna Filant; Parham Nejad; Anu Paul; Bridget Simonson; Srimeenakshi Srinivasan; Xuan Zhang; Leonora Balaj; Saumya Das; Roopali Gandhi; Louise C. Laurent; Anil K. Sood

doi:10.1007/978-1-4939-7652-2_5

Isolation of extracellular RNA from serum/plasma

Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang, Leonora Balaj, Saumya Das, Roopali Gandhi, Louise C. Laurent^*, Anil K. Sood

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Extracellular RNAs are initiating increased interest due to their potentials in serving as novel biomarkers, mediators of intercellular communication, and therapeutic applications. As a newly emerging field, one of the main obstacles is the lack of standardized protocols for RNA isolations. Here we describe protocols for commercially available kits that have been modified to yield consistent results for isolation of extracellular RNA from both whole serum/plasma and extracellular vesicle-enriched serum/plasma samples.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	43-57
Number of pages	15
DOIs	https://doi.org/10.1007/978-1-4939-7652-2_5
State	Published - 2018
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1740
ISSN (Print)	1064-3745

Keywords

Cell biology
Extracellular RNA
Extracellular Vesicle
Isolation
Molecular Biology
Nucleic acids
Purification and separation

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10.1007/978-1-4939-7652-2_5

Cite this

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abstract = "Extracellular RNAs are initiating increased interest due to their potentials in serving as novel biomarkers, mediators of intercellular communication, and therapeutic applications. As a newly emerging field, one of the main obstacles is the lack of standardized protocols for RNA isolations. Here we describe protocols for commercially available kits that have been modified to yield consistent results for isolation of extracellular RNA from both whole serum/plasma and extracellular vesicle-enriched serum/plasma samples.",

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AU - Nejad, Parham

AU - Paul, Anu

AU - Simonson, Bridget

AU - Srinivasan, Srimeenakshi

AU - Zhang, Xuan

AU - Balaj, Leonora

AU - Das, Saumya

AU - Gandhi, Roopali

AU - Laurent, Louise C.

AU - Sood, Anil K.

PY - 2018

Y1 - 2018

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AB - Extracellular RNAs are initiating increased interest due to their potentials in serving as novel biomarkers, mediators of intercellular communication, and therapeutic applications. As a newly emerging field, one of the main obstacles is the lack of standardized protocols for RNA isolations. Here we describe protocols for commercially available kits that have been modified to yield consistent results for isolation of extracellular RNA from both whole serum/plasma and extracellular vesicle-enriched serum/plasma samples.

KW - Cell biology

KW - Extracellular RNA

KW - Extracellular Vesicle

KW - Isolation

KW - Molecular Biology

KW - Nucleic acids

KW - Purification and separation

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

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Isolation of extracellular RNA from serum/plasma

Abstract

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