Isolation of CD3-, CD4-, CD8-, IL-2R+ thymocyte precursors by panning

Myra Small, Anis Sen Majumdar, Miriam Lieberman, Irving Weissman

Research output: Contribution to journalArticlepeer-review

Abstract

We present a panning method for isolation of thymocytes that are CD3-, CD4-, CD8- and IL-2R+. These cells have been isolated by positive selection on dishes coated with treatment with biotinylated 145-2C11, GK1.5, and 53-6.7 antibodies and negative selection on avidin coated dishes.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalJournal of Immunological Methods
Volume167
Issue number1-2
DOIs
StatePublished - 3 Jan 1994

Keywords

  • Lymphocyte
  • Planning
  • Precursor tyymocyte

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