Isolation of CD3-, CD4-, CD8-, IL-2R+ thymocyte precursors by panning

Myra Small; Anis Sen Majumdar; Miriam Lieberman; Irving Weissman

doi:10.1016/0022-1759(94)90079-5

Isolation of CD3^-, CD4^-, CD8^-, IL-2R⁺ thymocyte precursors by panning

Myra Small^*, Anis Sen Majumdar, Miriam Lieberman, Irving Weissman

^*Corresponding author for this work

Cell and Developmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

We present a panning method for isolation of thymocytes that are CD3^-, CD4^-, CD8^- and IL-2R⁺. These cells have been isolated by positive selection on dishes coated with treatment with biotinylated 145-2C11, GK1.5, and 53-6.7 antibodies and negative selection on avidin coated dishes.

Original language	English
Pages (from-to)	103-107
Number of pages	5
Journal	Journal of Immunological Methods
Volume	167
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(94)90079-5
State	Published - 3 Jan 1994

Keywords

Lymphocyte
Planning
Precursor tyymocyte

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10.1016/0022-1759(94)90079-5

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title = "Isolation of CD3-, CD4-, CD8-, IL-2R+ thymocyte precursors by panning",

abstract = "We present a panning method for isolation of thymocytes that are CD3-, CD4-, CD8- and IL-2R+. These cells have been isolated by positive selection on dishes coated with treatment with biotinylated 145-2C11, GK1.5, and 53-6.7 antibodies and negative selection on avidin coated dishes.",

keywords = "Lymphocyte, Planning, Precursor tyymocyte",

author = "Myra Small and Majumdar, {Anis Sen} and Miriam Lieberman and Irving Weissman",

note = "Funding Information: We thank Itzhak Oschry for FACS analysis and Libuse Jerabek for her assistance. Supported by USPHS grants CA 03352 and CA 42551.",

year = "1994",

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doi = "10.1016/0022-1759(94)90079-5",

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AU - Small, Myra

AU - Majumdar, Anis Sen

AU - Lieberman, Miriam

AU - Weissman, Irving

N1 - Funding Information: We thank Itzhak Oschry for FACS analysis and Libuse Jerabek for her assistance. Supported by USPHS grants CA 03352 and CA 42551.

PY - 1994/1/3

Y1 - 1994/1/3

N2 - We present a panning method for isolation of thymocytes that are CD3-, CD4-, CD8- and IL-2R+. These cells have been isolated by positive selection on dishes coated with treatment with biotinylated 145-2C11, GK1.5, and 53-6.7 antibodies and negative selection on avidin coated dishes.

AB - We present a panning method for isolation of thymocytes that are CD3-, CD4-, CD8- and IL-2R+. These cells have been isolated by positive selection on dishes coated with treatment with biotinylated 145-2C11, GK1.5, and 53-6.7 antibodies and negative selection on avidin coated dishes.

KW - Lymphocyte

KW - Planning

KW - Precursor tyymocyte

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ER -

Isolation of CD3^-, CD4^-, CD8^-, IL-2R⁺ thymocyte precursors by panning

Abstract

Keywords

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