Isolation of adult murine thymic stromal cells that naturally express Notch ligands

Myra Small

doi:10.1007/s00418-004-0747-0

Isolation of adult murine thymic stromal cells that naturally express Notch ligands

Myra Small

Cell and Developmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

This communication summarizes the procedures that enabled isolation of adult murine thymic stromal cell lines that naturally express Notch ligands Jagged-1 or Delta-1. Histochemical characterization of these cell lines, in terms of ligand and cell type, revealed epithelial cells that bear an antigen characteristic of the thymic medulla and express either Jagged-1 or Delta-1. FACS sorting of stromal cells that naturally express these and other ligands is thus feasible, and such cells can be used to investigate the activity of each ligand in Notch-mediated commitment to the T-lymphocyte pathway.

Original language	English
Pages (from-to)	513-516
Number of pages	4
Journal	Histochemistry and Cell Biology
Volume	123
Issue number	4-5
DOIs	https://doi.org/10.1007/s00418-004-0747-0
State	Published - Jun 2005

Keywords

Delta-1
Jagged-1
Notch ligands
Thymic stroma

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10.1007/s00418-004-0747-0

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title = "Isolation of adult murine thymic stromal cells that naturally express Notch ligands",

abstract = "This communication summarizes the procedures that enabled isolation of adult murine thymic stromal cell lines that naturally express Notch ligands Jagged-1 or Delta-1. Histochemical characterization of these cell lines, in terms of ligand and cell type, revealed epithelial cells that bear an antigen characteristic of the thymic medulla and express either Jagged-1 or Delta-1. FACS sorting of stromal cells that naturally express these and other ligands is thus feasible, and such cells can be used to investigate the activity of each ligand in Notch-mediated commitment to the T-lymphocyte pathway.",

keywords = "Delta-1, Jagged-1, Notch ligands, Thymic stroma",

author = "Myra Small",

note = "Funding Information: Acknowledgements Thanks to Jon Aster and Plavi Mittal for providing the ectodomain of Notch, to Willem van Ewijk for ER-TR5 antibody, and to Izhak Oschry for expert FACS operation. This work was supported by the Carl and Leonora Fingerhut Fund of Tel Aviv University.",

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N2 - This communication summarizes the procedures that enabled isolation of adult murine thymic stromal cell lines that naturally express Notch ligands Jagged-1 or Delta-1. Histochemical characterization of these cell lines, in terms of ligand and cell type, revealed epithelial cells that bear an antigen characteristic of the thymic medulla and express either Jagged-1 or Delta-1. FACS sorting of stromal cells that naturally express these and other ligands is thus feasible, and such cells can be used to investigate the activity of each ligand in Notch-mediated commitment to the T-lymphocyte pathway.

AB - This communication summarizes the procedures that enabled isolation of adult murine thymic stromal cell lines that naturally express Notch ligands Jagged-1 or Delta-1. Histochemical characterization of these cell lines, in terms of ligand and cell type, revealed epithelial cells that bear an antigen characteristic of the thymic medulla and express either Jagged-1 or Delta-1. FACS sorting of stromal cells that naturally express these and other ligands is thus feasible, and such cells can be used to investigate the activity of each ligand in Notch-mediated commitment to the T-lymphocyte pathway.

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Isolation of adult murine thymic stromal cells that naturally express Notch ligands

Abstract

Keywords

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