@article{e87fa09ffb3e46d5a3fe9a0c8e427db1,
title = "Isolation of adult murine thymic stromal cells that naturally express Notch ligands",
abstract = "This communication summarizes the procedures that enabled isolation of adult murine thymic stromal cell lines that naturally express Notch ligands Jagged-1 or Delta-1. Histochemical characterization of these cell lines, in terms of ligand and cell type, revealed epithelial cells that bear an antigen characteristic of the thymic medulla and express either Jagged-1 or Delta-1. FACS sorting of stromal cells that naturally express these and other ligands is thus feasible, and such cells can be used to investigate the activity of each ligand in Notch-mediated commitment to the T-lymphocyte pathway.",
keywords = "Delta-1, Jagged-1, Notch ligands, Thymic stroma",
author = "Myra Small",
note = "Funding Information: Acknowledgements Thanks to Jon Aster and Plavi Mittal for providing the ectodomain of Notch, to Willem van Ewijk for ER-TR5 antibody, and to Izhak Oschry for expert FACS operation. This work was supported by the Carl and Leonora Fingerhut Fund of Tel Aviv University.",
year = "2005",
month = jun,
doi = "10.1007/s00418-004-0747-0",
language = "אנגלית",
volume = "123",
pages = "513--516",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "4-5",
}