Isolation by polymerase chain reaction of a cDNA whose product partially complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells

Tal Teitz, Michal Penner, Dalia Eli, Merav Stark, Mary Bakhanashvili, Tova Naiman, Dan Canaani*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A xeroderma pigmentosum (XP) cell line from complementation group C has been complemented to attain ultraviolet (UV) resistance and DNA repair proficiency, by transfection with a human expression cDNA library, followed by selection to UV resistance. We now show that the transfected cDNAs can be rescued from cellular DNA of a secondary transformant by its in vitro amplification using expression-vector-specific oligodeoxyribonucleotides as primers in a polymerase chain reaction. The amplified cDNAs were cloned into a mammalian expression vector. Their transfection into XP cells identified a single cDNA which specifically complemented the UV sensitivity of a group C-derived cell line to the same partial UV-resistance levels exhibited by the transformant from which the cDNAs were rescued.

Original languageEnglish
Pages (from-to)295-298
Number of pages4
JournalGene
Volume87
Issue number2
DOIs
StatePublished - 15 Mar 1990

Funding

FundersFunder number
Mexican Government
Mexican Ministry of Public Education
National Council of Science and Technology
SESIC
Consejo Nacional de Ciencia y Tecnología

    Keywords

    • Recombinant DNA
    • expressible cDNA library
    • gene transfer
    • human disease
    • irradiation

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