TY - JOUR
T1 - Isolation and properties of the soluble c-type cytochromes of the dinoflagellate Peridinium Cinctum
AU - Hochman, Ayala
AU - Berman, Thomas
AU - Plotkin, Batya
AU - Schejter, Abel
N1 - Funding Information:
1 This research was partially supported by a grant from The National Council for Research and Development, Israel, and The K. F. K. Karlsruhe, Germany.
PY - 1985/11/15
Y1 - 1985/11/15
N2 - Four soluble cytochromes of the c type were isolated from the freshwater dinoflagellate Peridinium cinctum collected from Lake Kinneret, Israel. Cytochrome c with α-band maximum at 550 nm in the reduced state had a molecular mass of 10,200 Da, pI 7.4, and Em of 278 mV. This cytochrome was active in the respiratory chain of beef heart Keilin-Hartree particles. Cytochrome c-553 had a molecular mass of 13,200 Da, pI 4.9, and Em of 384 mV, and was active in light induced electron transport of Euglena gracilis chloroplast fragments. Cytochrome c-554 had a molecular mass of 13,500 Da, pI 4.4, and Em of 326 mV. This cytochrome was inactive in light induced electron transport but competed with cytochrome c-552 of Euglena in the assay. The acidic cytochrome c-557 was present in very small quantities. The properties of the soluble c-type cytochromes of P. cinctum are compatible with the classification of dinoflagellates as primitive eucaryotes.
AB - Four soluble cytochromes of the c type were isolated from the freshwater dinoflagellate Peridinium cinctum collected from Lake Kinneret, Israel. Cytochrome c with α-band maximum at 550 nm in the reduced state had a molecular mass of 10,200 Da, pI 7.4, and Em of 278 mV. This cytochrome was active in the respiratory chain of beef heart Keilin-Hartree particles. Cytochrome c-553 had a molecular mass of 13,200 Da, pI 4.9, and Em of 384 mV, and was active in light induced electron transport of Euglena gracilis chloroplast fragments. Cytochrome c-554 had a molecular mass of 13,500 Da, pI 4.4, and Em of 326 mV. This cytochrome was inactive in light induced electron transport but competed with cytochrome c-552 of Euglena in the assay. The acidic cytochrome c-557 was present in very small quantities. The properties of the soluble c-type cytochromes of P. cinctum are compatible with the classification of dinoflagellates as primitive eucaryotes.
UR - http://www.scopus.com/inward/record.url?scp=0022336985&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(85)90784-2
DO - 10.1016/0003-9861(85)90784-2
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AN - SCOPUS:0022336985
SN - 0003-9861
VL - 243
SP - 161
EP - 167
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -