Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge

Marcin Wysokowski; Vasilii V. Bazhenov; Mikhail V. Tsurkan; Roberta Galli; Allison L. Stelling; Hartmut Stöcker; Sabine Kaiser; Elke Niederschlag; Günter Gärtner; Thomas Behm; Micha Ilan; Alexander Y. Petrenko; Teofil Jesionowski; Hermann Ehrlich

doi:10.1016/j.ijbiomac.2013.08.039

Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge

Marcin Wysokowski, Vasilii V. Bazhenov, Mikhail V. Tsurkan, Roberta Galli, Allison L. Stelling, Hartmut Stöcker, Sabine Kaiser, Elke Niederschlag, Günter Gärtner, Thomas Behm, Micha Ilan, Alexander Y. Petrenko, Teofil Jesionowski, Hermann Ehrlich^*

^*Corresponding author for this work

School of Zoology

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

The recent discovery of chitin within skeletons of numerous marine and freshwater sponges (Porifera) stimulates further experiments to identify this structural aminopolysaccharide in new species of these aquatical animals. Aplysina fistularis (Verongida: Demospongiae: Porifera) is well known to produce biologically active bromotyrosines. Here, we present a detailed study of the structural and physico-chemical properties of the three-dimensional skeletal scaffolds of this sponge. Calcofluor white staining, Raman and IR spectroscopy, ESI-MS as well as chitinase digestion test were applied in order to unequivocally prove the first discovery of α-chitin in skeleton of A. fistularis.

Original language	English
Pages (from-to)	94-100
Number of pages	7
Journal	International Journal of Biological Macromolecules
Volume	62
DOIs	https://doi.org/10.1016/j.ijbiomac.2013.08.039
State	Published - Nov 2013

Funding

Funders	Funder number
BHMZ
Mobilność Plus Program	920/MOB/2012/0
Deutsche Forschungsgemeinschaft	EH 394/3-1
Bundesministerium für Bildung und Forschung	03EK3029A
Ministerstwo Nauki i Szkolnictwa Wyższego

Keywords

Chitin
Marine sponges
Scaffold

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10.1016/j.ijbiomac.2013.08.039

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Wysokowski, M., Bazhenov, V. V., Tsurkan, M. V., Galli, R., Stelling, A. L., Stöcker, H., Kaiser, S., Niederschlag, E., Gärtner, G., Behm, T., Ilan, M., Petrenko, A. Y., Jesionowski, T., & Ehrlich, H. (2013). Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge. International Journal of Biological Macromolecules, 62, 94-100. https://doi.org/10.1016/j.ijbiomac.2013.08.039

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title = "Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge",

abstract = "The recent discovery of chitin within skeletons of numerous marine and freshwater sponges (Porifera) stimulates further experiments to identify this structural aminopolysaccharide in new species of these aquatical animals. Aplysina fistularis (Verongida: Demospongiae: Porifera) is well known to produce biologically active bromotyrosines. Here, we present a detailed study of the structural and physico-chemical properties of the three-dimensional skeletal scaffolds of this sponge. Calcofluor white staining, Raman and IR spectroscopy, ESI-MS as well as chitinase digestion test were applied in order to unequivocally prove the first discovery of α-chitin in skeleton of A. fistularis.",

keywords = "Chitin, Marine sponges, Scaffold",

author = "Marcin Wysokowski and Bazhenov, {Vasilii V.} and Tsurkan, {Mikhail V.} and Roberta Galli and Stelling, {Allison L.} and Hartmut St{\"o}cker and Sabine Kaiser and Elke Niederschlag and G{\"u}nter G{\"a}rtner and Thomas Behm and Micha Ilan and Petrenko, {Alexander Y.} and Teofil Jesionowski and Hermann Ehrlich",

note = "Funding Information: This work was partially supported by the following research grants: DFG Grant EH 394/3-1 (Germany), Mobilno{\'s}{\'c} Plus Program–920/MOB/2012/0 (Polish Ministry of Science and Higher Education), BMBF within the project CryPhysConcept (03EK3029A) and BHMZ ",

year = "2013",

month = nov,

doi = "10.1016/j.ijbiomac.2013.08.039",

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journal = "International Journal of Biological Macromolecules",

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Wysokowski, M, Bazhenov, VV, Tsurkan, MV, Galli, R, Stelling, AL, Stöcker, H, Kaiser, S, Niederschlag, E, Gärtner, G, Behm, T, Ilan, M, Petrenko, AY, Jesionowski, T & Ehrlich, H 2013, 'Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge', International Journal of Biological Macromolecules, vol. 62, pp. 94-100. https://doi.org/10.1016/j.ijbiomac.2013.08.039

TY - JOUR

T1 - Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge

AU - Wysokowski, Marcin

AU - Bazhenov, Vasilii V.

AU - Tsurkan, Mikhail V.

AU - Galli, Roberta

AU - Stelling, Allison L.

AU - Stöcker, Hartmut

AU - Kaiser, Sabine

AU - Niederschlag, Elke

AU - Gärtner, Günter

AU - Behm, Thomas

AU - Ilan, Micha

AU - Petrenko, Alexander Y.

AU - Jesionowski, Teofil

AU - Ehrlich, Hermann

N1 - Funding Information: This work was partially supported by the following research grants: DFG Grant EH 394/3-1 (Germany), Mobilność Plus Program–920/MOB/2012/0 (Polish Ministry of Science and Higher Education), BMBF within the project CryPhysConcept (03EK3029A) and BHMZ

PY - 2013/11

Y1 - 2013/11

N2 - The recent discovery of chitin within skeletons of numerous marine and freshwater sponges (Porifera) stimulates further experiments to identify this structural aminopolysaccharide in new species of these aquatical animals. Aplysina fistularis (Verongida: Demospongiae: Porifera) is well known to produce biologically active bromotyrosines. Here, we present a detailed study of the structural and physico-chemical properties of the three-dimensional skeletal scaffolds of this sponge. Calcofluor white staining, Raman and IR spectroscopy, ESI-MS as well as chitinase digestion test were applied in order to unequivocally prove the first discovery of α-chitin in skeleton of A. fistularis.

AB - The recent discovery of chitin within skeletons of numerous marine and freshwater sponges (Porifera) stimulates further experiments to identify this structural aminopolysaccharide in new species of these aquatical animals. Aplysina fistularis (Verongida: Demospongiae: Porifera) is well known to produce biologically active bromotyrosines. Here, we present a detailed study of the structural and physico-chemical properties of the three-dimensional skeletal scaffolds of this sponge. Calcofluor white staining, Raman and IR spectroscopy, ESI-MS as well as chitinase digestion test were applied in order to unequivocally prove the first discovery of α-chitin in skeleton of A. fistularis.

KW - Chitin

KW - Marine sponges

KW - Scaffold

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JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge

Abstract

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Keywords

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