Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment

Ignacio Mastandrea, Divsha Sher, Prerna Magod, Dinorah Friedmann-Morvinski

Research output: Contribution to journalArticlepeer-review

Abstract

The immune fraction of the tumor microenvironment has been proven to play a fundamental role in glioblastoma progression and therapeutic response. Here, we present a detailed magnetic-bead-enrichment-based protocol to isolate and analyze the composition of this fraction from mouse brain tumors. The protocol is optimized to achieve high yields of viable immune cells. We also detail characterization of the immune subtypes by FACS analysis. Our procedure is applicable for either lentiviral-induced tumors or transplant models in syngeneic immunocompetent mice. For complete details on the use and execution of this protocol, please refer to Magod et al. (2021).

Original languageEnglish
Article number101106
JournalSTAR Protocols
Volume3
Issue number1
DOIs
StatePublished - 18 Mar 2022

Keywords

  • Cancer
  • Cell Biology
  • Cell isolation
  • Flow Cytometry/Mass Cytometry
  • Immunology
  • Model Organisms
  • Neuroscience

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