Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus

Cheryl L. Keen, Simona Mendelovitz, Gerald Cohen, Yair Aharonowitz, Kenneth L. Roy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5′ termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.

Original languageEnglish
Pages (from-to)172-176
Number of pages5
JournalMolecular Genetics and Genomics
Volume212
Issue number1
DOIs
StatePublished - Apr 1988

Keywords

  • Linear plasmid
  • Streptomyces clavuligerus
  • Terminal repeats

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