Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus

Cheryl L. Keen; Simona Mendelovitz; Gerald Cohen; Yair Aharonowitz; Kenneth L. Roy

doi:10.1007/BF00322461

Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus

Cheryl L. Keen, Simona Mendelovitz, Gerald Cohen, Yair Aharonowitz, Kenneth L. Roy^*

^*Corresponding author for this work

Biotechnology

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Abstract

A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5′ termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.

Original language	English
Pages (from-to)	172-176
Number of pages	5
Journal	Molecular Genetics and Genomics
Volume	212
Issue number	1
DOIs	https://doi.org/10.1007/BF00322461
State	Published - Apr 1988

Keywords

Linear plasmid
Streptomyces clavuligerus
Terminal repeats

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10.1007/BF00322461

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abstract = "A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5′ termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.",

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AU - Cohen, Gerald

AU - Aharonowitz, Yair

AU - Roy, Kenneth L.

PY - 1988/4

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KW - Linear plasmid

KW - Streptomyces clavuligerus

KW - Terminal repeats

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Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus

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Keywords

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