Isolation and characterization of a β-tubulin gene from Candida albicans

Herbert A. Smith, H. S. Allaudeen, Matthew H. Whitman, Yigal Koltin, Jessica A. German

Research output: Contribution to journalArticlepeer-review

Abstract

We report the isolation and nucleotide sequence determination of a β-tubulin gene (TUB2) from the pathogenic dimorphic fungus Candida albicans. Nucleotide sequence analysis revealed that TUB2 encodes a protein of 449 amino acids (aa) with considerable sequence homology to β-tubulins isolated from other fungal species. The nucleotide sequence of the C. albicans gene is 70% homologous to that of the Saccharomyces cerevisiae gene. The coding region for the C. albicansβ-tubulin gene is interrupted by two introns. The first intron occurs after the 4th aa and the second intron occurs after the 13th aa. A comparison with other fungal β-tubulin genes indicates that the intron locations are highly conserved. Codon usage in the C. albicans TUB2 gene is nonrandom, as has been observed for other fungal β-tubulhi genes. The C. albicans TUB2 gene is transcribed to yield a 1.8-kb mRNA species. On the basis of genomic Southern-blot analysis, we conclude that C. albicans most likely possesses a single β-tubulin gene.

Original languageEnglish
Pages (from-to)53-63
Number of pages11
JournalGene
Volume63
Issue number1
DOIs
StatePublished - 15 Mar 1988

Keywords

  • Recombinant DNA
  • conservation of intron positions
  • fungal genetics
  • sequence homologies

Fingerprint

Dive into the research topics of 'Isolation and characterization of a β-tubulin gene from Candida albicans'. Together they form a unique fingerprint.

Cite this