Isoelectric focusing in acrylamide gels: Use of amphoteric dyes as internal markers for determination of isoelectric points

Abigail Conway-Jacobs, Lawrence M. Lewin

Research output: Contribution to journalArticlepeer-review

Abstract

A procedure is described for determining the isoelectric points of proteins, utilizing isoelectric focusing in acrylamide gels containing amphoteric dyes as internal markers. With this method, the pI values of bovine serum albumin, ovalbumin, and bovine insulin were found to be 4.9, 4.7, and 5.3, respectively, in agreement with reported values obtained by other methods.

Original languageEnglish
Pages (from-to)394-400
Number of pages7
JournalAnalytical Biochemistry
Volume43
Issue number2
DOIs
StatePublished - Oct 1971

Fingerprint

Dive into the research topics of 'Isoelectric focusing in acrylamide gels: Use of amphoteric dyes as internal markers for determination of isoelectric points'. Together they form a unique fingerprint.

Cite this