Ischemic tolerance conferred to cultured rat neurons by heat shock is not mediated by opening of adenosine triphosphate-sensitive potassium channels

Ayelet Reshef, Noam Di Capua, Oded Sperling, Esther Zoref-Shani*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of sublethal heat shock on the capacity of neurons to resist subsequent ischemia-reperfusion-induced cell injury, was studied in a model of primary rat neuronal cultures, subjected to chemical ischemia. Exposure of the cultures to sublethal heat shock (42°C; 20 min) resulted in elevation in cellular content of heat shock protein (HSP)-70, at 4 h following the shock, and in acquisition of a 15 h 'time window of protection' against ischemia-reperfusion insult, with maximum protection at 4 h. Presence in the culture medium of glibenclamide (2 μM), a blocker of ATP sensitive potassium (K(ATP)) channels, did not abolish the acquisition of protection throughout the entire duration of the acquired 'time window of protection'. The results demonstrate that heat shock induces in neurons a protective mechanism against ischemia-reperfusion insult, probably associated with enhanced expression of HSPs, which does not depend on opening of K(ATP) channels. In this respect, the neuronal 'heat-shock mechanism' for the acquisition of ischemic tolerance differs from the neuronal 'adenosine mechanism' and probably also from the heart 'heat shock mechanism' for the acquisition of protection. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)223-226
Number of pages4
JournalNeuroscience Letters
Volume287
Issue number3
DOIs
StatePublished - 30 Jun 2000

Funding

FundersFunder number
Heller Fund for Medical Research of the Sackler Faculty of Medicine
Tel Aviv University

    Keywords

    • Adenosine
    • Adenosine triphosphate-sensitive potassium channels
    • Glibenclamide
    • Heat shock
    • Heat shock protein
    • Ischemia-reperfusion damage
    • Ischemic tolerance
    • Neuronal cultures

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