Is the Phytophthora citrophthora culture filtrate a reliable tool for the in vitro selection of resistant Citrus variants?

A. Vardi*, E. Epstein, A. Breiman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Nucellar calli from four Citrus cultivars with known resistance to the Phytophthora citrophthora pathogen were chosen as experimental material to test the pathogen's response to culture filtrate (CF). Sensitivity of the four calli to CF of the fungus was in reverse order to what is known on the susceptibility of the cultivars in vivo. Sensitivity of protoplasts derived from the same four calli to 2,4-dichlorophenoxyacetic acid (2,4-D) was in the same order as that of calli to CF. Protoplasts derived from calli selected for tolerance to CF showed a higher plating efficiency with increasing concentration of CF in the medium. TLC and GLC determinations showed the presence of indole acetic acid in the culture filtrate. Results indicate that CF of P. citrophthora cannot be used as a selection tool in vitro.

Original languageEnglish
Pages (from-to)569-574
Number of pages6
JournalTheoretical And Applied Genetics
Volume72
Issue number4
DOIs
StatePublished - Jul 1986

Keywords

  • Citrus
  • Culture-filtrate
  • Indole acetic acid
  • Phytophthora citrophthora
  • Protoplast

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